|
| Status |
Public on Nov 20, 2023 |
| Title |
WT_C1 |
| Sample type |
SRA |
| |
|
| Source name |
Seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
cell type: Seedlings
genotype: wild type
treatment: control
time: 0 hour
|
| Treatment protocol |
Wild type and atdamt1 seedlings were heat treated for 2.5 hours
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was harvested using Trizol. mRNA was purified by oligo-dT beads
RNA libraries for mRNA-seq were prepared using NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina following manufacturer's protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Description |
10-day-old wild type seedling mRNAs were used to generate the cDNA libraries, respectively, according to NEBNext UltraTM II Directional RNA Library Prep Kit for Illumina
|
| Data processing |
Sequence reads were trimmed for adaptor sequence/low-quality sequence using cutadapter
Trimmed sequence reads were mapped to TAIR10 using STAR
Differential expression analysis was performed by DESeq2
Assembly: TAIR10
Supplementary files format and content: Excel files include RPKM values for each Sample.
|
| |
|
| Submission date |
Jul 13, 2023 |
| Last update date |
Nov 20, 2023 |
| Contact name |
Chongsheng He |
| E-mail(s) |
chongshenghe@outlook.com
|
| Phone |
+8619198053436
|
| Organization name |
Hunan university
|
| Street address |
27 Tianma road
|
| City |
Changsha |
| State/province |
Hunan |
| ZIP/Postal code |
410082 |
| Country |
China |
| |
|
| Platform ID |
GPL21785 |
| Series (1) |
| GSE237262 |
METTL4-mediated N6-methyladenine DNA modification regulates thermotolerance in Arabidopsis thaliana |
|
| Relations |
| BioSample |
SAMN36437311 |
| SRA |
SRX21006039 |
