|
Status |
Public on Aug 02, 2023 |
Title |
798_1_S95, silk gland_rep 1 |
Sample type |
SRA |
|
|
Source name |
Silk gland
|
Organism |
Bombyx mori |
Characteristics |
tissue: Silk gland strain: A798 silk gland weight phenotype_1: 1.441 g silk gland weight phenotype_2: 1.189 g
|
Growth protocol |
Silkworms of each strain were fed at the same regulated feeding conditions (25℃ and 71% humidity) up to day 5 of the fifth larval instar.
|
Extracted molecule |
total RNA |
Extraction protocol |
The total RNA was extracted from the silk gland using TRIzol (Invitrogen, Carlsbad, CA, USA). 1 µg of total RNA was used for the construction of sequencing libaries. The RNA libraries were validated according to the manufacturer’s instructions.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
798_1
|
Data processing |
The original data were analyzed using fastp version 0.19.5, and clean data with Q30 > 92.93% was obtained from each sample Compare clean data(reads) with reference genome. Use assembly software StringTie Version 1.3.3b for reference-based assembly. The differentially expressed genes were determined at |log2 (fold change)| > 1 and p-adjust < 0.05 using DESeq2 Version 1.24.0 Assembly: StringTie Supplementary files format and content: tab-delimited text file includes raw counts for each Sample Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
|
|
|
Submission date |
Jul 26, 2023 |
Last update date |
Aug 02, 2023 |
Contact name |
Sun Lindan |
E-mail(s) |
sunlindan@ujs.edu.cn
|
Phone |
13675118671
|
Organization name |
Jiangsu University
|
Department |
School of Life and Sciences
|
Street address |
301 xuefu road
|
City |
Zhenjiang |
ZIP/Postal code |
212013 |
Country |
China |
|
|
Platform ID |
GPL23127 |
Series (1) |
GSE239380 |
Identification of genes associated with the development of silk glands using transcriptomics in silkworm (Bombyx mori) |
|
Relations |
BioSample |
SAMN36719186 |
SRA |
SRX21167728 |