|
| Status |
Public on Aug 02, 2011 |
| Title |
NT09/296 Normal_Colon (1699538181_B) |
| Sample type |
RNA |
| |
|
| Source name |
Colon Tissue
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
sample type: patient Sample
disease: colorectal cancer
|
| Treatment protocol |
Not Applicable
|
| Growth protocol |
Cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% heat inactivated fetal bovine serum (HIFBS) (Gibco, Invitrogen) and 100U/ml penicillin and streptomycin (Gibco, Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells using RNeasy Mini Kit (Qiagen) with modifications. Cells were first washed with PBS twice and then lysed in TRIzol (Invitrogen) before purification using RNA extraction columns and buffers provided in the kit. The RNA concentration was quantified using Nanodrop (Thermo Scientific). 500ng total RNA was used for cRNA amplification using Illumina TotalPrep RNA Amplification kit (Ambion). Steps were carried out by following the instructional manual in the kit.
|
| Label |
Streptavidin-Cy3
|
| Label protocol |
Standard Illumina label protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Scanner used: Illumina BeadArray Reader
Scanning protocol: Scan factor of 1, PMT525
Software: Illumina Beadscan version 3.0
|
| Description |
1699538181_B
|
| Data processing |
Data is extracted using Illumina Genomestudio version 2010.2, Expression Module version 1.7.0
Data were analyzed using Partek GS6.4 Ver 6.09.0922.
Normalization algorithm: RMA
Analysis was performed using ANOVA.
|
| |
|
| Submission date |
Aug 02, 2011 |
| Last update date |
Aug 24, 2011 |
| Contact name |
Sherry Wang |
| E-mail(s) |
bchdm@nus.edu.sg
|
| Organization name |
National University of Singapore
|
| Department |
Biochemistry
|
| Street address |
21 Lower Kent Ridge Road
|
| City |
Singapore |
| ZIP/Postal code |
119077 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL14186 |
| Series (1) |
| GSE24993 |
Expression Profiling of Tissues versus Primary Cultures from Colorectal Cancer Patients |
|
