|
| Status |
Public on May 28, 2024 |
| Title |
32D-MPL-Calr(ins5) 1 |
| Sample type |
RNA |
| |
|
| Source name |
cell line generated from bone marrow of C3H mice
|
| Organism |
Mus musculus |
| Characteristics |
cell type: murine 32D
tissue: bone marrow
lineage: myeloid
transduced protein: MPL, Calr(ins5)
|
| Treatment protocol |
32D cells were transduced with MPL and Calr (WT or ins5). Cells were starved from IL3 over night in RPMI+FCS+P/S.
|
| Growth protocol |
cells were cultured at 37°C, 5% CO2, in RPMI supplemented with 10% FCS, 1% P/S and 5ng/ml IL3
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cell were washed with PBS to remove any IL3 and cultured over night. Cell were harvested from overnight culture and total RNA was isolated using QIAzol method.
|
| Label |
Biotin
|
| Label protocol |
We used the Affymetrix WT Pico kit for cDNA synthesis, amplification, cRNA synthesis, second-cycle cDNA synthesis, as well as fragmentation and labeling with Biotin according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Labeled fragments were hybridized to Applied Biosystems Clariom S Mouse Arrays for 16h at 45°C with 60 rpm in an Affymetrix Hybridization Oven 645.
|
| Scan protocol |
After washing and staining, the arrays were scanned with the Affymetrix GeneChip Scanner 3000 7G. CEL-files were produced from the raw data with Affymetrix GeneChip Command Console Software.
|
| Description |
Gene expression from WT mouse CXCL12-abundant reticular cells
|
| Data processing |
CEL files were analysed using the oligo R package. RMA method, log2 transformed were used to normalized the probe intensities.
|
| |
|
| Submission date |
Aug 18, 2023 |
| Last update date |
May 28, 2024 |
| Contact name |
Geoffroy Andrieux |
| Organization name |
University clinics Freiburg
|
| Street address |
Breisacherstr 153
|
| City |
Freiburg |
| ZIP/Postal code |
79110 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23038 |
| Series (2) |
| GSE241162 |
Oncogenic Calreticulin Induces Immune Escape by Stimulating TGF-β Expression and Regulatory T Cell Expansion in the Bone Marrow Microenvironment - microarray |
| GSE241365 |
Oncogenic Calreticulin Induces Immune Escape by Stimulating TGF-β Expression and Regulatory T Cell Expansion in the Bone Marrow Microenvironment |
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