|
Status |
Public on Jan 15, 2014 |
Title |
ChIP-Seq analysis of H3K4me3 in human CD15 cells; Lib EpiLabSPL Row 1797 |
Sample type |
SRA |
|
|
Source name |
flow sorted CD15+ cells from cord blood; Lib EpiLabSPL Row 1797
|
Organism |
Homo sapiens |
Characteristics |
sample alias: 28.0 sample common name: CD15 Primary Cells molecule: genomic DNA disease: none biomaterial_provider: FHCRC biomaterial_type: Primary Cell cell_type: CD15 Primary Cells markers: CD15+ donor_id: 23.0 donor_age: NA donor_health_status: none donor_sex: Male donor_ethnicity: NA passage_if_expanded: NA chip_protocol_bead_type: Protein A-Sepharose 4B chip_antibody: H3K4me3 chip_protocol_chromatin_amount: 10 million cells extraction_protocol: SDS lysis chip_protocol_antibody_amount: 2-15 ug chip_protocol_bead_amount: 70 ul bed volume chip_protocol: Bernstein_BROAD_ENCODE_protocol extraction_protocol_sonication_cycles: 1 chip_antibody_catalog: 07-473 chip_antibody_provider: Millipore chip_antibody_lot: DAM1623866 experiment_type: Histone H3K4me3 extraction_protocol_type_of_sonicator: Bioruptor
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Library construction protocol: Single read - Illumina
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina Genome Analyzer IIx |
|
|
Description |
sample_term_id: CL_0000775 assay_term_id: OBI_0000716 nucleic_acid_term_id: SO_0000352 Design description: Human Chromatin IP REMC Sequencing on Illumina Library name: Lib EpiLabSPL Row 1797 EDACC Genboree Experiment Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.8043 EDACC Genboree Sample Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.2562 **************** For data usage terms and conditions, please refer to: http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies ****************
|
Data processing |
Various levels of processed data files will be made available as this project proceeds.
|
|
|
Submission date |
Aug 03, 2011 |
Last update date |
May 15, 2019 |
Contact name |
BROAD INSTITUTE |
E-mail(s) |
rharris1@bcm.tmc.edu
|
Organization name |
Broad Institute
|
Street address |
-
|
City |
Cambridge |
State/province |
MA |
ZIP/Postal code |
02142 |
Country |
USA |
|
|
Platform ID |
GPL10999 |
Series (1) |
GSE17312 |
BI Human Reference Epigenome Mapping Project |
|
Relations |
SRA |
SRX088697 |
BioSample |
SAMN00012132 |