|
| Status |
Public on Dec 28, 2011 |
| Title |
ChIP-Seq analysis of WCE in human HUES64 derived CD184+ cells; DNA_Lib 244 |
| Sample type |
SRA |
| |
|
| Source name |
CD184+ endoderm differentiated in vitro from stem cells; DNA_Lib 244
|
| Organism |
Homo sapiens |
| Characteristics |
cell_type: CD184+ endoderm cultured cells
sample alias: 263
sample common name: hESC Derived CD184+ Endoderm Cultured Cells
lineage: NA
medium: RPMI 100ng/mL Activin A 50ng/mL Wnt3a
molecule: genomic DNA
disease: presumed normal
passage: 1
line: HUES64 derived CD184+
differentiation_method: in vitro culture
batch: BioSAli 137
biomaterial_type: Cell Line
differentiation_stage: differentiated
Sex: Male
biomaterial_provider: Harvard University
extraction_protocol_sonication_cycles: 1
chip_protocol_chromatin_amount: standard
extraction_protocol: cell/nuclei lysis
experiment_type: ChIP-Seq Input
chip_protocol: Input
extraction_protocol_type_of_sonicator: Branson
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
RANDOM |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
sample_term_id: CL_0000223
assay_term_id: OBI_0000716
nucleic_acid_term_id: SO_0000352
Design description: Human Chromatin IP REMC Sequencing on Illumina
Library name: DNA_Lib 244
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.8669
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.9460
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
|
| Data processing |
**********************************************************************
ANALYSIS FILE NAME: GSM772979_BI.hESC_Derived_CD184+_Endoderm_Cultured_Cells.Input.DNA_Lib_244.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DNA_Lib 244-DNA_Lib 244-DNA_Lib 244-DNA_Lib 244-DNA_Lib 244.hg19.level.1.release.5
ANALYSIS TITLE: Mapping of hESC Derived CD184+ Endoderm Cultured Cells ChIP-Seq Input Data
ANALYSIS DESCRIPTION: Illumina reads produced by ChIP-Seq Input on hESC Derived CD184+ Endoderm Cultured Cells, Donor DNA_Lib 244, were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.10738
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
ALIGNMENT_POSTPROCESSING: None
READ_EXTENSION: 200bp
RELEASE_NUMBER: Human Epigenome Atlas 5
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 30,922,630
NUMBER_OF_Input_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 56
FINDPEAKS_SCORE: 0.0051
FINDPEAKS_PERCENTILE: 46
HOTSPOT_SCORE: 0.0103
HOTSPOT_PERCENTILE: 51
IROC_SCORE: 1.0
IROC_PERCENTILE: 100
POISSON_SCORE: 0.057
POISSON_PERCENTILE: 52
MAXIMUM_REPLICATE_CORRELATION: 0.96
**********************************************************************
ANALYSIS FILE NAME: GSM772979_BI.hESC_Derived_CD184+_Endoderm_Cultured_Cells.Input.DNA_Lib_244.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: DNA_Lib 244-DNA_Lib 244-DNA_Lib 244-DNA_Lib 244-DNA_Lib 244.hg19.level.2.release.5
ANALYSIS TITLE: Raw Signal Density Graphs of hESC Derived CD184+ Endoderm Cultured Cells ChIP-Seq Input Data
ANALYSIS DESCRIPTION: Illumina ChIP-Seq Input read mappings from hESC Derived CD184+ Endoderm Cultured Cells, Donor DNA_Lib 244, were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.11002
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 200bp
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
GENOMIC_WINDOW: 20bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 5
BROWSER_TRACK_NAME: hDCE Input 44
BROWSER_TRACK_DESCRIPTION: BI hESC Derived CD184+ Endoderm Cultured Cells ChIP-Seq Input Library DNA_Lib 244 EA Release 5
QUALITY SCORES:
NUMBER_OF_MAPPED_READS: 30,922,630
NUMBER_OF_Input_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 56
FINDPEAKS_SCORE: 0.0051
FINDPEAKS_PERCENTILE: 46
HOTSPOT_SCORE: 0.0103
HOTSPOT_PERCENTILE: 51
IROC_SCORE: 1.0
IROC_PERCENTILE: 100
POISSON_SCORE: 0.057
POISSON_PERCENTILE: 52
MAXIMUM_REPLICATE_CORRELATION: 0.96
**********************************************************************
|
| |
|
| Submission date |
Aug 03, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
BROAD INSTITUTE |
| E-mail(s) |
rharris1@bcm.tmc.edu
|
| Organization name |
Broad Institute
|
| Street address |
-
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE17312 |
BI Human Reference Epigenome Mapping Project |
|
| Relations |
| SRA |
SRX088894 |
| BioSample |
SAMN00691473 |
| Named Annotation |
GSM772979_BI.hESC_Derived_CD184+_Endoderm_Cultured_Cells.Input.DNA_Lib_244.wig.gz |
