|
| Status |
Public on Dec 21, 2011 |
| Title |
B10:LJ110 del pdhr MMAcetat |
| Sample type |
RNA |
| |
|
| Source name |
LJ110 del pdhr MMAcetat
|
| Organism |
Escherichia coli |
| Characteristics |
background strain: LJ110
genetic modification: PdhR knockout mutant (LJ110deltapdhR)
|
| Growth protocol |
MMAcetat
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAeasy
|
| Label |
Cy3
|
| Label protocol |
200 ng of total RNA were applied for Cy3-labelling reaction using the MessageAmp II-Bacteria Kit according to supplier´s recommendation (Ambion). As a result of IVT (in vitro transcription) reaction using aminoallyl-dUTP antisense aRNA were generated and subsequently coupled with fluorescent dye Cy3.
|
| |
|
| Hybridization protocol |
Labeled cRNA was hybridized to Agilent´s E.coli 8x15k microarrays for 16h at 68°C
|
| Scan protocol |
Agilent DNA Microarray Scanner with default settings.
|
| Description |
B10:LJ110 del pdhr MMAcetat
|
| Data processing |
Expression values were calculated by the software package Feature Extraction 10.5.1.1 using protocol GE1_105_Dec08 (Agilent Technologies; Waldbronn, Germany)
|
| |
|
| Submission date |
Aug 11, 2011 |
| Last update date |
Dec 21, 2011 |
| Contact name |
Robert Geffers |
| E-mail(s) |
robert.geffers@helmholtz-hzi.de
|
| Phone |
+49 531-6181-3058
|
| Organization name |
HCI - Helmholtz Centre for Infection Research
|
| Department |
Dep. Molecular Bacteriology
|
| Lab |
Genome Analytics
|
| Street address |
Inhoffenstr. 7
|
| City |
Braunschweig |
| ZIP/Postal code |
38124 |
| Country |
Germany |
| |
|
| Platform ID |
GPL13359 |
| Series (1) |
| GSE31333 |
Elucidation and validation of new targets of the global regulator PdhR in Escherichia coli |
|
