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Sample GSM7775053 Query DataSets for GSM7775053
Status Public on Sep 12, 2023
Title CSF_Control_1
Sample type RNA
 
Source name Control CSF 1
Organism Homo sapiens
Characteristics tissue: Adipocyte-dervied Extracellular Vesicles_CSF
disease state: Control
Extracted molecule total RNA
Extraction protocol adipocyte-derived small extracellular vesicles were isolated from serum and CSF using Exoquick and Exoquick TC precipitation reagents followed by immunoselection. Total RNA was extracted using mirVana RNA isolation kits. Total RNA was amplified with the complete Seramir Exosome RNA amplification kit.
Label Biotin
Label protocol 1,000 ng of RNA was labeled using Affymetrix FlashTag Biotin HSR RNA labeling kit.
 
Hybridization protocol Following labeling RNA was hybridized to affymetrix miRNA 4.0 GeneChips for 16 hours and washed and stained on the GeneChip Fluidics Station 450 using protocol FS450_0002.
Scan protocol GeneChips were scanned on the Affymetrix GeneChip Scanner 3000
Description miRNA expression data
Data processing Resulting data were normalized in Affymetrix Expression Console software using RMA-DABG and exported into Partek Genomics Suite version 6.6 for analysis.
intensity signals after normalization with RMA-DABG
 
Submission date Sep 11, 2023
Last update date Sep 12, 2023
Contact name Rachael Batabyal
Organization name Children's National Hospital
Street address 111 Michigan Ave NW
City Washington
ZIP/Postal code 20010
Country USA
 
Platform ID GPL19117
Series (1)
GSE242923 miRNA expression profile from Adipocyte-derived Small Extracellular Vesicles from the Serum and cerebrospinal fluid of Alzheimer Disease Patients and Controls

Data table header descriptions
ID_REF
VALUE RMA-DABG normalized signal

Data table
ID_REF VALUE
MIMAT0000001_st 0.81
MIMAT0015091_st 0.96
MIMAT0000002_st 0.92
MIMAT0015092_st 0.89
MIMAT0020301_st 1.02
MIMAT0000003_st 1.31
MIMAT0020302_st 1.07
MIMAT0000004_st 0.64
MIMAT0000005_st 1.28
MIMAT0015093_st 1.76
MIMAT0020303_st 0.55
MIMAT0000006_st 1.47
MIMAT0020304_st 0.85
MIMAT0000007_st 1.04
MIMAT0015094_st 1.32
MIMAT0000008_st 1.04
MIMAT0020305_st 1.01
MIMAT0000009_st 8.09
MIMAT0020306_st 0.92
MIMAT0000010_st 0.81

Total number of rows: 36222

Table truncated, full table size 731 Kbytes.




Supplementary file Size Download File type/resource
GSM7775053_Sample4.CEL.gz 827.5 Kb (ftp)(http) CEL

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