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Sample GSM7776910 Query DataSets for GSM7776910
Status Public on Sep 13, 2023
Title rat#15-control_cortex_7d
Sample type RNA
Source name brain tissue
Organism Rattus norvegicus
Characteristics animal: rat#15
Sex: male
strain: Wistar
treatment: TBI
tissue: brain
brain region: cortex
activation agent: PBS treatment
time: 7 days
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with RNeasy Mini Kit followed by DNase I treatment.
Label SYBR Green
Label protocol cDNA was synthesized using RevertAid First Strand cDNA Synthesis Kit (TermoFisher) by corresponding standard protocol. The PCRs set with qPCRmix-HS SYBR master mixes (Evrogen, Russia) were carried out in a Bio-Rad cfx96 (BioRad, USA) in the following mode: primary denaturation 95 °C for 5 min; denaturation 95 °C 20 s, annealing 56–63 °C 20 s, elongation 72 °C 20 s (40 cycles).
Hybridization protocol n/a
Scan protocol n/a
Description control
Data processing For the normalization it uses the average of housekeeping genes: GAPDH, ACTB.
Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)
Fold Change worksheet reports control/test (median all samples PBS treatment/ median all samples EV treatment) ratios at 7 and 14 days for brain regions (cortex, striatum, hippocampus).
Genes that did not show expression were excluded.
Submission date Sep 12, 2023
Last update date Sep 13, 2023
Contact name Diana Salikhova
Organization name RUDN University
Department Institute of molecular and cellular medecine
Lab Laboratory of cellular biotechnology
Street address Miklukho-Maklaya, 8
City Moscow
ZIP/Postal code 117198
Country Russia
Platform ID GPL33755
Series (1)
GSE242996 Real-time quantitative PCR analysis of rat's brain mRNAs

Data table header descriptions
VALUE normalized signal

Data table
A01 0.048138444
A02 0.086002527
B01 0.001103779
B02 9.01904E-05
B03 0.001285608
B04 0.000467479
E01 2.25778E-06
E02 0.035731328
F01 0.005806675
G01 0.000355795
G02 1.85058E-05
G03 0.007238969
G04 0.190342109
H01 0.002788755
H02 0.000607754
H03 0.420448208
H04 0.238710401
H05 0.002281547
H06 0.002649618
H07 0.009643342

Total number of rows: 20

Table truncated, full table size <1 Kbytes.

Supplementary data files not provided

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