|
| Status |
Public on Sep 01, 2011 |
| Title |
β-catenin transgenic thymocytes |
| Sample type |
RNA |
| |
|
| Source name |
β-catenin transgenic thymocytes
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: β-catenin transgenic
age: 16 wks
tissue: thymocytes
|
| Growth protocol |
Primary thymocytes were harvested and isolated by using a 40um cell strainer, and resuspended in RPMI 1640 medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen Rneasy Plus according to manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100-300ng total RNA (Affymetrix GeneChip® Whole Transcript (WT) Sense Target Labeling Assay)
|
| |
|
| Hybridization protocol |
Following fragmentation,2.5 ug of cRNA were hybridized for 16-18 hr at 45C on Affymetrix Mouse Gene ST 1.0 Array. The arrays were washed and stained in the Affymetrix Fluidics Station 450 using FS450_0007 script.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G and the CEL intensity files were produced by GeneChip Operating Software v. 1.4.
|
| Description |
b-Cat Tg
gene expression data from β-catenin transgenic thymocytes
|
| Data processing |
Normalized expression values were produced using RMA normalization according the default settings in Partek Genomics Suite
|
| |
|
| Submission date |
Aug 14, 2011 |
| Last update date |
Sep 01, 2011 |
| Contact name |
ruiqing wang |
| E-mail(s) |
rwang@coh.org
|
| Organization name |
city of hope
|
| Street address |
1500 duarte rd
|
| City |
duarte |
| State/province |
ca |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE31371 |
Expression data from Thymocytes of WT, TCF-1-deficient and β-catenin transgenic mice |
|
