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| Status |
Public on Sep 01, 2024 |
| Title |
mimics_NC2 |
| Sample type |
SRA |
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| Source name |
cell line
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| Organism |
Homo sapiens |
| Characteristics |
tissue: cell line
cell line: A549
cell type: Lung cancer cells
genotype: WT
treatment: Transfected with mimics NC
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from peripheral blood plasma using an EZ-press Serum/Plasma RNA Purification Kit (EZB Bioscience, #EZB-RN2).
RNA samples were treated with 3'-terminal ligation, reverse transcription primer hybridization, 5'-terminal ligation, cDNA single strand synthesis, library amplification
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Description |
mimics_NC2
mRNA_raw_counts.txt
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| Data processing |
Blastn (2.9.0+) software was used to identify piRNAs and obtain the read count number quantitatively
According to the CPM value of each sample, violin and density charts were made to show the distribution of the CPM of each sample
Assembly: Ensembl_v107
Supplementary files format and content: mRNA FPKM values for each sample
Supplementary files format and content: piRNA CPM values for each sample
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| Submission date |
Sep 22, 2023 |
| Last update date |
Sep 01, 2024 |
| Contact name |
Chuandong Wang |
| E-mail(s) |
wangchuandong@yeah.net
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| Organization name |
SJTU
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| Street address |
320 Yueyang Road
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platform ID |
GPL24676 |
| Series (1) |
| GSE243794 |
PiRNA DQ571591 regulated the function of non-small cell lung cancer cells |
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| Relations |
| BioSample |
SAMN37513348 |
| SRA |
SRX21858663 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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