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Sample GSM7812894 Query DataSets for GSM7812894
Status Public on Oct 02, 2023
Title FBP1 OE MDA-MB231
Sample type RNA
 
Source name MDA-MB231 cells
Organism Homo sapiens
Characteristics cell line: MDA-MB-231
transfection: FBP1 overexpression
Treatment protocol Cells were transfected with FBP1 expression vector or empty vector as control.
Growth protocol MDA-MB231 cells were cultivated in DMEM with 10% FCS and pen/strep in the presence of 5% CO2 at 37° C .
Extracted molecule total RNA
Extraction protocol RNA extraction was done by triazole/chloroform follwoing established protocols (Sigma-Aldrich). RNA was extracted from 500.000 cells. RNA concentrations were adjusted to 100ng/µl. Quality control with Agilent 2100.
Label Cy3
Label protocol standard Illumina protocol
 
Hybridization protocol standard Illumina protocol (Human-HT12-V4 Expression BeadChip)
Scan protocol According to manufacturer with BeadArray Reader (Illumina).
Description SAMPLE 1
FBP1
Data processing quantile normalization with all samples
 
Submission date Sep 28, 2023
Last update date Oct 02, 2023
Contact name Stefan Wolfl
E-mail(s) wolfl@uni-hd.de
Organization name Heidelberg University, Heidelberg, Germany
Department Institute of Pharmacy and Molecular Biotechnology
Lab Pharm. Biology and Bioanalytics
Street address Im Neuenheimer Feld 364
City Heidelberg
ZIP/Postal code D-69120
Country Germany
 
Platform ID GPL10558
Series (1)
GSE244288 FBP1 overexpression in MDA-MB-231 breast cancer cells

Data table header descriptions
ID_REF
VALUE quantile normalized (mean value)
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1343048 15048.18942 1.79513E-27
ILMN_1343049 13089.68092 3.81176E-24
ILMN_1343050 361.6260432 3.29029E-28
ILMN_1343052 367.6223876 3.74382E-29
ILMN_1343059 78.98731182 0.799247867
ILMN_1343061 3413.552457 3.27256E-25
ILMN_1343062 9304.790118 3.8754E-38
ILMN_1343063 199.878771 5.4624E-21
ILMN_1343064 382.8065861 2.86034E-19
ILMN_1343291 13266.91194 6.42465E-42
ILMN_1343295 4071.850538 1.7844E-25
ILMN_1343296 76.98262625 0.900907817
ILMN_1343297 78.19669097 0.850685307
ILMN_1343298 86.57586534 0.232475914
ILMN_1343299 86.45087598 0.164323932
ILMN_1343300 86.07718629 0.265403866
ILMN_1343301 86.62842727 0.104740611
ILMN_1343302 83.63883278 0.113993229
ILMN_1343303 79.57385279 0.732999982
ILMN_1343304 82.25673373 0.561427771

Total number of rows: 48107

Table truncated, full table size 1727 Kbytes.




Supplementary file Size Download File type/resource
GSM7812894_FBP1_OE_MDA-MB231_Grn.idat.gz 1.6 Mb (ftp)(http) IDAT

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