|
Status |
Public on Oct 02, 2023 |
Title |
FBP1 OE MDA-MB231 |
Sample type |
RNA |
|
|
Source name |
MDA-MB231 cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: MDA-MB-231 transfection: FBP1 overexpression
|
Treatment protocol |
Cells were transfected with FBP1 expression vector or empty vector as control.
|
Growth protocol |
MDA-MB231 cells were cultivated in DMEM with 10% FCS and pen/strep in the presence of 5% CO2 at 37° C .
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA extraction was done by triazole/chloroform follwoing established protocols (Sigma-Aldrich). RNA was extracted from 500.000 cells. RNA concentrations were adjusted to 100ng/µl. Quality control with Agilent 2100.
|
Label |
Cy3
|
Label protocol |
standard Illumina protocol
|
|
|
Hybridization protocol |
standard Illumina protocol (Human-HT12-V4 Expression BeadChip)
|
Scan protocol |
According to manufacturer with BeadArray Reader (Illumina).
|
Description |
SAMPLE 1 FBP1
|
Data processing |
quantile normalization with all samples
|
|
|
Submission date |
Sep 28, 2023 |
Last update date |
Oct 02, 2023 |
Contact name |
Stefan Wolfl |
E-mail(s) |
wolfl@uni-hd.de
|
Organization name |
Heidelberg University, Heidelberg, Germany
|
Department |
Institute of Pharmacy and Molecular Biotechnology
|
Lab |
Pharm. Biology and Bioanalytics
|
Street address |
Im Neuenheimer Feld 364
|
City |
Heidelberg |
ZIP/Postal code |
D-69120 |
Country |
Germany |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE244288 |
FBP1 overexpression in MDA-MB-231 breast cancer cells |
|