|
| Status |
Public on Oct 03, 2023 |
| Title |
0-week control - 2521 |
| Sample type |
SRA |
| |
|
| Source name |
Whole Lung
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Whole Lung
genotype: C57BL6/J
treatment: Control
|
| Treatment protocol |
Mice underwent treatment with either L-NAME (0.5 g/L) in the water and high fat diet (60% lipid content) for 2 and 5 weeks, compared to 0 week controls.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After sacrifice, lungs were immediately taken and flash frozen. RNA was isolated using Rneasy kit (Qiagen).
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Partek Flow platform used for data processing
Tophat 2 was used to align reads to the mm10 genome build
Reads were quantified to mm10 using the Partek E/M annotation model
Reads were normalized to counts per million (CPM) with 0.0001 added to the result.
Assembly: mm10
Supplementary files format and content: Text file including CPM for each sample (in columns) and gene (in rows)
|
| |
|
| Submission date |
Sep 28, 2023 |
| Last update date |
Oct 03, 2023 |
| Contact name |
James West |
| E-mail(s) |
j.west@vanderbilt.edu
|
| Organization name |
Vanderbilt University
|
| Department |
Medicine
|
| Lab |
Pulmonary
|
| Street address |
4200 E 9TH Ave, Box B133
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37201 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE244304 |
Myeloid Cell Derived IL1b Contributes to Pulmonary Vascular Remodeling in Heart Failure with Preserved Ejection Fraction |
|
| Relations |
| BioSample |
SAMN37596228 |
| SRA |
SRX21924245 |
