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| Status |
Public on Oct 03, 2023 |
| Title |
Myeloid Cell Derived IL1b Contributes to Pulmonary Vascular Remodeling in Heart Failure with Preserved Ejection Fraction |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Goals and objectives of this study were to determine by bulk RNA-sequencing transcriptomic changes that occur in the lungs of a murine model of heart failure with preserved ejection fraction caused by administration of high fat diet and eNOS inhibitor L-NAME.
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| Overall design |
Four C57/BL6 mice each were given either a combination of high fat diet and L-NAME or control (low fat diet with no L-NAME) for 5 weeks. Lungs were isolated after sacrifice, enzymatically digested, separately labeled with hashing antibodies, and submitted for single cell RNA sequencing.
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| Contributor(s) |
Agrawal V, West J |
| Citation(s) |
37929582 |
| |
| Submission date |
Sep 28, 2023 |
| Last update date |
Jan 02, 2024 |
| Contact name |
James West |
| E-mail(s) |
j.west@vanderbilt.edu
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| Organization name |
Vanderbilt University
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| Department |
Medicine
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| Lab |
Pulmonary
|
| Street address |
4200 E 9TH Ave, Box B133
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37201 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1022149 |
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