|
| Status |
Public on Jan 17, 2024 |
| Title |
HH_2.1.2 vs HMHC_3.7 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
HH_2.1-2
|
| Organism |
Haloferax mediterranei |
| Characteristics |
growth condition: nitrogen starvation (120 h)
strain: HM26
|
| Growth protocol |
Hfx. mediterranei strains were grown in different culture media at 42 °C containing 25% (w/v) salt water (25% SW) (Rodriguez-Valera et al., 1983). The pH of all media was adjusted to 7.2-7.4, with NaOH. Hfx. mediterranei cells were grown in a defined medium with 40 mM NH4Cl and 10 g/L glucose supplemented with 0.0005 g/L FeCl3, 0.5 g/L KH2PO4, 100 mM MOPS, and 50 µg/mL uracil until the mid-exponential phase. Subsequently, cells were harvested by centrifugation for 10 min at 13000 g, washed twice with 25% SW, and transferred to a medium without nitrogen or carbon source.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from these samples by using RNeasy Mini Kit (Qiagen) following product specifications
|
| Label |
Cy5
|
| Label protocol |
Two-Color Microarray-Based Prokaryote Analysis Fair Play III Labeling v. 1.3 (Agilent)
|
| |
|
| Channel 2 |
| Source name |
HMHC_3.7
|
| Organism |
Haloferax mediterranei |
| Characteristics |
growth condition: carbon starvation (120 h)
strain: HM26
|
| Growth protocol |
Hfx. mediterranei strains were grown in different culture media at 42 °C containing 25% (w/v) salt water (25% SW) (Rodriguez-Valera et al., 1983). The pH of all media was adjusted to 7.2-7.4, with NaOH. Hfx. mediterranei cells were grown in a defined medium with 40 mM NH4Cl and 10 g/L glucose supplemented with 0.0005 g/L FeCl3, 0.5 g/L KH2PO4, 100 mM MOPS, and 50 µg/mL uracil until the mid-exponential phase. Subsequently, cells were harvested by centrifugation for 10 min at 13000 g, washed twice with 25% SW, and transferred to a medium without nitrogen or carbon source.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from these samples by using RNeasy Mini Kit (Qiagen) following product specifications
|
| Label |
Cy3
|
| Label protocol |
Two-Color Microarray-Based Prokaryote Analysis Fair Play III Labeling v. 1.3 (Agilent)
|
| |
|
| |
| Hybridization protocol |
Microarrays where hybridized and washed according to manufacturer's protocol
|
| Scan protocol |
Scanned on an Agilent G2505C scanner.
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
| |
|
| Submission date |
Oct 04, 2023 |
| Last update date |
Jan 17, 2024 |
| Contact name |
María José Antonio Bonete |
| E-mail(s) |
mjbonete@ua.es
|
| Organization name |
Universidad de Alicante
|
| Department |
Agroquímica y Bioquímica
|
| Street address |
Carretera San Vicente del Raspeig s/n
|
| City |
Sant Vicent del Raspeig |
| State/province |
Alicante |
| ZIP/Postal code |
03690 |
| Country |
Spain |
| |
|
| Platform ID |
GPL18892 |
| Series (1) |
| GSE244624 |
Analysis of Lsm protein-mediated regulation in the haloarchaeon Haloferax mediterranei |
|
