|
Status |
Public on Jul 07, 2024 |
Title |
39627 Umbilical Artery, Control |
Sample type |
SRA |
|
|
Source name |
Umbilical Artery
|
Organism |
Macaca mulatta |
Characteristics |
tissue: Umbilical Artery sample: 39627 cell type: Endothelial cells treatment: Control
|
Extracted molecule |
polyA RNA |
Extraction protocol |
RNA was isolated using RNeasy Micro kit (Qiagen) and quality was assessed using Agilent 2100 Bioanalyzer with RNA 6000 Pico chip by Oregon Health & Science University (OHSU) Gene Profiling Shared Resource. All samples had a RIN score above 5.0. NovaSeq short read sequencing assays (1 x 100 bp) were performed by the OHSU Massively Parallel Sequencing Shared Resource.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
ARTERY_ALL_THC_vs_Control.csv
|
Data processing |
Raw data were checked for quality using FastQC, trimmed using Trimmomatic, aligned using STAR, analyzed for differential gene expression using DESeq2 in R. Pathway analysis was performed with STRING . Assembly: Mmul_10 Supplementary files format and content: .csv files are the normalized counts following DESeq2 differential gene expression analysis Supplementary files format and content: NA
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|
|
Submission date |
Oct 04, 2023 |
Last update date |
Jul 07, 2024 |
Contact name |
Hillary Le |
E-mail(s) |
lehi@ohsu.edu
|
Organization name |
Oregon Health & Science University
|
Street address |
3303 S Bond Ave
|
City |
PORTLAND |
State/province |
OR |
ZIP/Postal code |
97239 |
Country |
USA |
|
|
Platform ID |
GPL27943 |
Series (1) |
GSE244644 |
In utero exposure of delta-9-tetrahydrocannabinol (THC) affects vascular transcriptome in rhesus macaques |
|
Relations |
BioSample |
SAMN37686177 |
SRA |
SRX21991866 |