|
| Status |
Public on Jul 07, 2024 |
| Title |
39625 Fetal Aorta, THC |
| Sample type |
SRA |
| |
|
| Source name |
Fetal Aorta
|
| Organism |
Macaca mulatta |
| Characteristics |
tissue: Fetal Aorta
sample: 39625
cell type: Endothelial cells
treatment: THC
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was isolated using RNeasy Micro kit (Qiagen) and quality was assessed using Agilent 2100 Bioanalyzer with RNA 6000 Pico chip by Oregon Health & Science University (OHSU) Gene Profiling Shared Resource.
All samples had a RIN score above 5.0. NovaSeq short read sequencing assays (1 x 100 bp) were performed by the OHSU Massively Parallel Sequencing Shared Resource.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
AORTA_ALL_THC_vs_Control.csv
|
| Data processing |
Raw data were checked for quality using FastQC,
trimmed using Trimmomatic,
aligned using STAR,
analyzed for differential gene expression using DESeq2 in R.
Pathway analysis was performed with STRING .
Assembly: Mmul_10
Supplementary files format and content: .csv files are the normalized counts following DESeq2 differential gene expression analysis
Supplementary files format and content: NA
|
| |
|
| Submission date |
Oct 04, 2023 |
| Last update date |
Jul 07, 2024 |
| Contact name |
Hillary Le |
| E-mail(s) |
lehi@ohsu.edu
|
| Organization name |
Oregon Health & Science University
|
| Street address |
3303 S Bond Ave
|
| City |
PORTLAND |
| State/province |
OR |
| ZIP/Postal code |
97239 |
| Country |
USA |
| |
|
| Platform ID |
GPL27943 |
| Series (1) |
| GSE244644 |
In utero exposure of delta-9-tetrahydrocannabinol (THC) affects vascular transcriptome in rhesus macaques |
|
| Relations |
| BioSample |
SAMN37686161 |
| SRA |
SRX21991882 |
