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Status |
Public on Jun 04, 2012 |
Title |
EndometrialStromalCell_Normal_Eutopic_1-H2O2 |
Sample type |
RNA |
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Source name |
eutopic endometrial stromal cell from patients without endometriosis, treated with H2O2
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Organism |
Homo sapiens |
Characteristics |
cell type: eutopic endometrial stromal age: 35 cycle phase: early proliferative diagnosis: mature cystic teratoma and leiomyoma r-afs stage(score): -
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Treatment protocol |
After 24 hours of incubation, medium was exchanged with Phenol Red-free DMEM(Sigma D5921) + 1uM hydrogen peroxide + 200IU/ml potassium benzylpenicillin(Meiji) + 200ug/ml streptomycin sulfate(Meiji) + 2.5mg/ml amphotericin B(Sigma A2942). After 60 hours of incubation, RNA was collected. Supplementary hydrogen peroxide was added to medium every 48 hours. Control cells were incubated in Phenol Red-free DMEM(Sigma D5921) + 200IU/ml potassium benzylpenicillin(Meiji) + 200ug/ml streptomycin sulfate(Meiji) + 2.5mg/ml amphotericin B(Sigma A2942) for 60 hours and its RNA was collected.
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Growth protocol |
Endometrial stromal cells were incubated in DMEM (Sigma D5921) + 10% FBS (BioWest) + 200IU/ml potassium benzylpenicillin (Meiji) + 200ug/ml streptomycin sulfate (Meiji) + 2.5mg/ml amphotericin B (Sigma A2942) for 24 hours.
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Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using RNeasy Mini(QIAGEN).
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Label |
Cy3
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Label protocol |
According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling), Version 5.7, March 2008).
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Hybridization protocol |
According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling), Version 5.7, March 2008).
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Scan protocol |
According to the manufacturer’s protocol (One-Color Microarray-Based Gene Expression Analysis (Quick Amp Labeling), Version 5.7, March 2008, Agilent Scanner Settings).
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Data processing |
Microarray data were analyzed following import into GeneSpring 11.5 software (Agilent Technologies) Threshold: 1.0 Logbase: 2 Technology: Agilent.SingleColor.14850 Normalization: Shift to 75 percentile Baseline Transformation: None
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Submission date |
Aug 18, 2011 |
Last update date |
Jun 04, 2012 |
Contact name |
Shinya Toyokuni |
E-mail(s) |
akatsuka@med.nagoya-u.ac.jp
|
Organization name |
Nagoya University
|
Department |
Pathology
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Street address |
65 Tsuruma-Cho, Showa-Ku
|
City |
Nagoya |
State/province |
Aichi |
ZIP/Postal code |
466-8550 |
Country |
Japan |
|
|
Platform ID |
GPL6480 |
Series (1) |
GSE31515 |
Transcriptome analysis of endometriotic stromal cells treated with hydrogen peroxide |
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