|
| Status |
Public on Oct 31, 2011 |
| Title |
A549_premiR-375_12h |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
trasfected with premiR-375 in A549
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
transfection time: 12h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA), followed by treatment with DNase I. Double-stranded cDNA was synthesized from 500 ng of total RNA using MMLV-RT and poly dT primer incorporating the T7 promoter.
|
| Label |
Cy5
|
| Label protocol |
Cy5-sample cRNA and Cy3-sample cRNA were generated and hybridized to a Whole Human Genome oligo DNA microarray kit (Agilent Technologies) with 41000 distinct probes.
|
| |
|
| Channel 2 |
| Source name |
trasfected with premiR-NC in A549
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A549
transfection time: 12h
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy kit (Qiagen, Valencia, CA), followed by treatment with DNase I. Double-stranded cDNA was synthesized from 500 ng of total RNA using MMLV-RT and poly dT primer incorporating the T7 promoter.
|
| Label |
Cy3
|
| Label protocol |
Cy5-sample cRNA and Cy3-sample cRNA were generated and hybridized to a Whole Human Genome oligo DNA microarray kit (Agilent Technologies) with 41000 distinct probes.
|
| |
|
| |
| Hybridization protocol |
Cy5-sample cRNA and Cy3-sample cRNA were generated and hybridized to a Whole Human Genome oligo DNA microarray kit (Agilent Technologies) with 41000 distinct probes.
|
| Scan protocol |
Whole Human Genome oligo DNA microarray with 41000 distinct probes was scanned using an Agilent DNA microarray scanner (G2505B, Agilent Technologies). Images were quantified using Agilent Feature Extraction Software.
|
| Data processing |
LOWESS normalized, background subtracted VALUE data obtained from ratio of processed Red signal/processed Green signal using Agilent Feature Extraction Software (version A.7.5.1).
|
| |
|
| Submission date |
Aug 22, 2011 |
| Last update date |
Oct 31, 2011 |
| Contact name |
Osada Hirotaka |
| E-mail(s) |
hosada@aichi-cc.jp
|
| Phone |
+81-52-764-2993
|
| Fax |
+81-52-763-5233
|
| Organization name |
Aichi Cancer Center Research Institute
|
| Lab |
Division of Molecular Oncology
|
| Street address |
1-1 Kanokoden, Chikusa-ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
464-8681 |
| Country |
Japan |
| |
|
| Platform ID |
GPL6480 |
| Series (1) |
| GSE31566 |
Pre-miR-375_A549 vs. Pre-miR-NC#2 (Ambion) _A549 |
|
