|
Status |
Public on Aug 01, 2024 |
Title |
Control for C26 B |
Sample type |
RNA |
|
|
Source name |
muscle tissue
|
Organism |
Mus musculus |
Characteristics |
strain: CD2F1 tumor status: non tumor bearing Sex: male
|
Treatment protocol |
1x10^6 C26, or 5x10^5 C-26 and LLC cells were injected into mice. Tamoxifen (1mg/10g) was injected IP into KPP mice to induce tumor formation
|
Growth protocol |
All mouse experiments were approved the Medical University of South Carolina Institutional Animal Care and Use Committee under protocol IACUC-2021-01223. Animals were housed with littermates, up to 5 total mice/cage and given ad libitum access to a standard show diet and water. Due to sexual dimorphisms in cancer cachexia, only male mice were used in this study
|
Extracted molecule |
total RNA |
Extraction protocol |
TA muscles were isolated from sacrificed mice and flash frozen
|
Label |
no label
|
Label protocol |
No label
|
|
|
Hybridization protocol |
No hyb
|
Scan protocol |
No scan
|
Description |
Total RNA extracted from muscle tissue, 100ng loaded onto the platform
|
Data processing |
Each tumor model were isolated individually on nCounter platform. Background and Normalization to housekeeping genes was performed in nCounter software. Counts were extracted and converted to z-scores
|
|
|
Submission date |
Oct 12, 2023 |
Last update date |
Aug 01, 2024 |
Contact name |
Benjamin Pryce |
E-mail(s) |
bpryce89@gmail.com
|
Phone |
8434787926
|
Organization name |
Medical University of South Carolina
|
Street address |
173 Ashley Ave
|
City |
Charleston |
ZIP/Postal code |
29425 |
Country |
USA |
|
|
Platform ID |
GPL20885 |
Series (1) |
GSE245208 |
nCounter Mouse Inflammation (V2) Panel on Muscle from Cancer Cachexia Models |
|