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Sample GSM7838686 Query DataSets for GSM7838686
Status Public on Aug 01, 2024
Title Tumor Bearing for LLC B
Sample type RNA
 
Source name muscle tissue
Organism Mus musculus
Characteristics strain: C57/B6
tumor status: tumor bearing
Sex: male
Treatment protocol 1x10^6 C26, or 5x10^5 C-26 and LLC cells were injected into mice. Tamoxifen (1mg/10g) was injected IP into KPP mice to induce tumor formation
Growth protocol All mouse experiments were approved the Medical University of South Carolina Institutional Animal Care and Use Committee under protocol IACUC-2021-01223. Animals were housed with littermates, up to 5 total mice/cage and given ad libitum access to a standard show diet and water. Due to sexual dimorphisms in cancer cachexia, only male mice were used in this study
Extracted molecule total RNA
Extraction protocol TA muscles were isolated from sacrificed mice and flash frozen
Label no label
Label protocol No label
 
Hybridization protocol No hyb
Scan protocol No scan
Description Total RNA extracted from muscle tissue, 100ng loaded onto the platform
Data processing Each tumor model were isolated individually on nCounter platform. Background and Normalization to housekeeping genes was performed in nCounter software. Counts were extracted and converted to z-scores
 
Submission date Oct 12, 2023
Last update date Aug 01, 2024
Contact name Benjamin Pryce
E-mail(s) bpryce89@gmail.com
Phone 8434787926
Organization name Medical University of South Carolina
Street address 173 Ashley Ave
City Charleston
ZIP/Postal code 29425
Country USA
 
Platform ID GPL20885
Series (1)
GSE245208 nCounter Mouse Inflammation (V2) Panel on Muscle from Cancer Cachexia Models

Data table header descriptions
ID_REF
VALUE Normalized Processed signal

Data table
ID_REF VALUE
Ager 20
Alox12 20
Alox15 20
Alox5 20
Areg 20
Arg1 20
Atf2 311.86
Bcl2l1 82
Bcl6 141.14
Birc2 188.19
C1qa 104.85
C1qb 122.32
C1ra 43.01
C1s 295.73
C2 26.88
C3 930.19
C3ar1 68.55
C4a 158.62
C6 20
C7 150.55

Total number of rows: 266

Table truncated, full table size 2 Kbytes.




Supplementary file Size Download File type/resource
GSM7838686_20200115_208706840421_LLC_B_11.RCC.gz 3.1 Kb (ftp)(http) RCC

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