|
| Status |
Public on Sep 10, 2011 |
| Title |
BYZ1_RNA-seq |
| Sample type |
SRA |
| |
|
| Source name |
Yeast S. cerevisiae strain BYZ1
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
strain: BYZ1(MATa/MATalpha his3delta1/his3delta1 leu2delta0/leu2delta0 lys2delta0/+ met15delta0/+ ura3delta0/ura3delta0)
application: Labortory strain
|
| Growth protocol |
Yeast cells were cultured in 25mL YPD medium (1g/L yeast extract, 2g/L peptone, and 2g/L glucose) with an initial OD600 of 0.05.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted at early-stationary phase by the hot phenol method and then treated with RNase-free DNase I (TaKaRa) for 30 min according to the manufacturer’s protocols. The poly (A) containing mRNA molecules were purified and the cDNA libraries were prepared according to the manufacturer’s instructions (Illumina). The cDNA library products were sequenced on the Illumina HiSeq™ 2000.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
sacCer2
|
| Data processing |
After removing reads containing sequencing adapters and reads of low quality (the percentage of the low quality bases of quality value </= 5 is more than 50% in a read), the clear reads were aligned to the S. cerevisiae S288c genes (ftp://ftp.ncbi.nih.gov/genomes/Fungi/Saccharomyces_cerevisiae_uid128) and genes using SOAPAligner.Mismatches no more than 2 bases were allowed in the alignment. Alignment file build: sacCer2
|
| |
|
| Submission date |
Aug 23, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Daoqiong Zheng |
| E-mail(s) |
zhengdaoqiong@163.com
|
| Phone |
86 571 88206636
|
| Organization name |
College of Life Sciences, Zhejiang University
|
| Department |
Institute of Microbiology
|
| Street address |
866 Yuhangtang Road
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platform ID |
GPL13821 |
| Series (1) |
| GSE31601 |
Transcription profile analysis of S. cerevisiae strains |
|
| Relations |
| SRA |
SRX093263 |
| BioSample |
SAMN00714191 |
