|
| Status |
Public on Oct 26, 2023 |
| Title |
BLA_FearRecall_fr11_B2_S1178 |
| Sample type |
SRA |
| |
|
| Source name |
Brain_Basolateral_Amygdala
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Brain_Basolateral_Amygdala
genotype: TRAP2 Ai14
treatment: Fear recall
|
| Treatment protocol |
Fear conditioning paradigm
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
OligodT primers were used to enrich mRNA
Brain tissues were dissociated with Worthington Papain Digestion Kit RNA libraries were prepared using smart seq 3
Novaseq 6000 was used for sequencing with standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Smartseq
|
| Data processing |
Fastq reads were mapped with STAR(2.7.9a)
Assembly: mm10
Supplementary files format and content: Cell by gene matrix format
|
| |
|
| Submission date |
Oct 24, 2023 |
| Last update date |
Oct 26, 2023 |
| Contact name |
Wenfei Sun |
| E-mail(s) |
Wenfei-sun@stanford.edu
|
| Organization name |
Stanford University
|
| Street address |
318 Campus Dr.
|
| City |
Stanford |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE246147 |
Single cell RNAseq of brian cells in the basolateral amygdala with fear conditioning |
|
| Relations |
| BioSample |
SAMN37972518 |
| SRA |
SRX22208632 |
