|
Status |
Public on Mar 07, 2013 |
Title |
H3Cter |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
H3Cter ChIP DNA from cells harvested 1h after meiosis induction
|
Organism |
Schizosaccharomyces pombe |
Characteristics |
antibody: H3Cter antibody vendor: Abcam
|
Growth protocol |
Cells in a pat1-114 background were induced to enter meiosis by the haploid meiosis system, and harvested one hour after the induction.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Cells were crosslinked with formaldehyde and ChIPs were performed as previously described (Yamada et al., EMBO J 23: 1792-803) except using following antibodies. Anti-H3K9ac and -H3K14ac antibodies were purchased from Millipore. Anti-H3K4me3, and -H3 antibodies were from Abcam.
|
Label |
biotin
|
Label protocol |
Amplified DNA were labeled with biotin as described (Katou et al., Nature 424:1078-83)
|
|
|
Channel 2 |
Source name |
Input DNA from cells harvested 1h after meiosis induction
|
Organism |
Schizosaccharomyces pombe |
Characteristics |
antibody: none
|
Growth protocol |
Cells in a pat1-114 background were induced to enter meiosis by the haploid meiosis system, and harvested one hour after the induction.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Cells were crosslinked with formaldehyde and ChIPs were performed as previously described (Yamada et al., EMBO J 23: 1792-803) except using following antibodies. Anti-H3K9ac and -H3K14ac antibodies were purchased from Millipore. Anti-H3K4me3, and -H3 antibodies were from Abcam.
|
Label |
biotin
|
Label protocol |
Amplified DNA were labeled with biotin as described (Katou et al., Nature 424:1078-83)
|
|
|
|
Hybridization protocol |
16 h at 45C using a hybridization oven 640 (affymetrix) as described in the Affymetrix Chromatin Immunoprecipitation Assay Protocol (P/N 702238)
|
Scan protocol |
Fluidics station 400 protocol EukGE-WS2v5 (affymetrix) was performed to wash and stain arrays. The arrays were scanned on GeneChip Scanner 3000 7G (affymetrix).
|
Description |
H3Cter / WCE, meiosis 1h
|
Data processing |
Data were quantile normalized and smoothed with Affymetrix Tiling Analysis Software
|
|
|
Submission date |
Aug 25, 2011 |
Last update date |
Mar 07, 2013 |
Contact name |
Shintaro YAMADA |
E-mail(s) |
ss107140@mail.ecc.u-tokyo.ac.jp
|
Organization name |
University of Tokyo
|
Street address |
3-8-1 Komaba
|
City |
Meguro-ku |
State/province |
Tokyo |
ZIP/Postal code |
153-8902 |
Country |
Japan |
|
|
Platform ID |
GPL7715 |
Series (1) |
GSE31648 |
Histone H3 lysine9 acetylation, rather than lysine4 trimethylation, marks meiotic recombination hotspots and promotes recombination initiation in fission yeast |
|