|
| Status |
Public on Nov 24, 2023 |
| Title |
Testes KO at P1 2 |
| Sample type |
SRA |
| |
|
| Source name |
Testes
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Testes
genotype: Osteocalcin KO
|
| Growth protocol |
Mice with one day of life were sacrificed by decapitation and the tissues (testes or hippocampus) were dissecated and freeze immediately in liquid nitrogen for porterior RNA isolation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvasted using the Rneasy mini kit plus (Qiagen)
RNA libraries for RNA-seq were generated using NEBNext Ultra™ RNA Library Prep Kit for Illumina
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
GD004
|
| Data processing |
Sequence reads were trimmed using Trimgalore version 0.6.6 to exclude low-quality bases.
Trimmed sequence reads were mapped to GRCm39/mm39 using STAR version 2.7.8a.
Gene expression levels were determined by measuring the number of reads mapped to each gene region using RSEM version 1.3.3.
Assembly: mm39
Supplementary files format and content: tab-delimited text file raw counts for each Sample
|
| |
|
| Submission date |
Nov 19, 2023 |
| Last update date |
Nov 24, 2023 |
| Contact name |
Kazuya Tokumura |
| Organization name |
Gifu Pharmaceutical University
|
| Department |
Bioactive Molecules
|
| Lab |
Pharmacology
|
| Street address |
1-25-4 Daigakunishi
|
| City |
Gifu |
| ZIP/Postal code |
501-1196 |
| Country |
Japan |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE248211 |
Osteocalcin of maternal and embryonic origins synergize to establish homeostasis in offspring |
|
| Relations |
| BioSample |
SAMN38322417 |
| SRA |
SRX22575849 |
