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Status
Public on Sep 07, 2011
Title
preT.DN3B.Th#1
Sample type
RNA
Source name
thymus
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Filter/syringe plunger teasing, ACK lysis Lin+ magnetic depletion (Dynobeads), 2 rounds of sorting, 2d sort directly into trizol
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
preT.DN3B.Th#1, DN3b thymocytes
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Sep 06, 2011
Last update date
Jul 21, 2021
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE180556
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
16.3167
10344622
673.3537
10344624
481.6166
10344633
753.4716
10344637
199.3774
10344653
19.0742
10344658
121.7258
10344674
10.0934
10344679
36.4182
10344707
275.2319
10344713
1595.6609
10344723
543.3964
10344725
52.5619
10344741
5344.7415
10344743
25.7864
10344750
352.2018
10344772
28.1897
10344789
291.0373
10344797
235.312
10344799
393.3864
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM791149_EA07068_110600_MoGene_T.DN3B.TH_1.CEL.gz
4.7 Mb
(ftp) (http) CEL
Processed data included within Sample table
