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Status
Public on Sep 07, 2011
Title
preT.DN3B.Th#3
Sample type
RNA
Source name
thymus
Organism
Mus musculus Characteristics
age: 6 weeks
Extracted molecule
total RNA
Extraction protocol
Filter/syringe plunger teasing, ACK lysis Lin+ magnetic depletion (Dynobeads), 2 rounds of sorting, 2d sort directly into trizol
Label
Biotin
Label protocol
Affy GeneChip WT cDNA Synthesis kit/cRNA Cleanup kit
Hybridization protocol
Affy 1.0 ST Hyb Kit
Scan protocol
Affymetrix GeneChip Scanner
Description
preT.DN3B.Th#3, DN3b thymocytes
Data processing
The datasets were pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
Submission date
Sep 06, 2011
Last update date
Jul 21, 2021
Contact name
Liang Yang
Organization name
Harvard Medical School
Department
Pathology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02115
Country
USA
Platform ID
GPL6246 Series (1)
Relations Reanalyzed by
GSE180556
Data table header descriptions ID_REF VALUE RMA normalized signal intensity
Data table ID_REF VALUE 10344620
18.0504
10344622
480.9863
10344624
398.5805
10344633
773.8387
10344637
197.7794
10344653
16.6336
10344658
47.252
10344674
12.2353
10344679
34.6708
10344707
80.4427
10344713
2551.8815
10344723
589.6879
10344725
52.0046
10344741
1941.8549
10344743
22.9733
10344750
215.7922
10344772
27.6469
10344789
116.7853
10344797
285.0851
10344799
165.3182
24922 422 Kbytes .Supplementary file Size Download File type/resource GSM791151_EA07068_110659_MoGene_T.DN3B.TH_3.CEL.gz
4.2 Mb
(ftp) (http) CEL
Processed data included within Sample table
