|
| Status |
Public on Feb 08, 2024 |
| Title |
WT Chromatin MNase 0.75 U |
| Sample type |
SRA |
| |
|
| Source name |
WT chromatin
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
genotype: Wildtype
treatment: MNase 0.75 U
|
| Treatment protocol |
After spheroplast and nuclei isolation chromatin was extracted and digested with 0.75 U of MNase.
|
| Growth protocol |
Both wildtype and triple knockout strains were grown at 30◦C in YPD media upto OD-2 to 2.5.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA was extracted by using phenol: chloroform
Library was prepared as per protocol described in Bowman et al., BMC Genomics (2013)
|
| |
|
| Library strategy |
MNase-Seq |
| Library source |
genomic |
| Library selection |
MNase |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Using Bowtie aligner2 the sequenced paired-end reads were mapped to the Saccharomyces cerevisiae reference genome((as described in earlier Mieczkowski et al., 2016)
Supplementary files format and content: biwig
|
| |
|
| Submission date |
Dec 09, 2023 |
| Last update date |
Feb 08, 2024 |
| Contact name |
Ajaz ul Hamid Wani |
| E-mail(s) |
ahwani@kashmiruniversity.ac.in
|
| Phone |
9797918365
|
| Organization name |
University of Kashmir
|
| Department |
Biotechnology
|
| Lab |
Genome Organization
|
| Street address |
Hazratbal
|
| City |
Srinagar |
| State/province |
Jammu and Kashmir |
| ZIP/Postal code |
190006 |
| Country |
India |
| |
|
| Platform ID |
GPL17342 |
| Series (1) |
| GSE220880 |
MNase titration-Seq of Saccharomyces cerevisiae. |
|
| Relations |
| BioSample |
SAMN38747761 |
| SRA |
SRX22842312 |
