|
| Status |
Public on Dec 16, 2023 |
| Title |
Skin normal 37 |
| Sample type |
genomic |
| |
|
| Source name |
Saliva
|
| Organism |
Homo sapiens |
| Characteristics |
gender: F
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Extraction of genomic DNA from saliva was performed using MagMAX DNA Multi-Sample Ultra 2.0 Kit on KingFisher™ Flex Purification System.
|
| Label |
Cy3, Cy5
|
| Label protocol |
250ng of genomic DNA was whole-genome amplified in an overnight reaction at 37˚C using amplification master mix (MA1, MA2 and MSM). After incubation the amplified DNA was fragmented with fragmentation mix (FMS), precipitated with isopropanol and precipitation mix (PM1) and resuspended in hybridization buffer (RA1).
|
| |
|
| Hybridization protocol |
RA1 resuspended DNA was loaded onto Infinium Asian Screening Array-24 v1.0 BeadChip . After incubation 16 - 24 hours at 48˚C, single-base extension and allele-specific staining was performed on a Te-flow chamber rack system (Tecan, Maennedorf, Switzerland).
|
| Scan protocol |
After allele-specific staining the Beadchip was coated with XC4, dried for 1 hour and scanned by IScan Software (Illumina).
|
| Description |
Genomic DNA extracted from saliva was genotyped usingInfinium Asian Screening Array-24 v1.0 BeadChip).
gst-skin-37
|
| Data processing |
Genomic DNA extracted from saliva was genotyped using ASA-24 v1.0 Beadchip (Illumina).
|
| |
|
| Submission date |
Dec 11, 2023 |
| Last update date |
Dec 16, 2023 |
| Contact name |
Tham H Hoang |
| E-mail(s) |
tham.hoang@uconn.edu
|
| Organization name |
Vingroup Big Data Institute
|
| Street address |
Floor 9th, 458 Minh Khai
|
| City |
Hanoi |
| ZIP/Postal code |
000000 |
| Country |
Viet Nam |
| |
|
| Platform ID |
GPL33106 |
| Series (1) |
| GSE248483 |
A study of genetic variants associated with skin traits in the Vietnamese population |
|
