|
| Status |
Public on Feb 09, 2024 |
| Title |
H3K27me3_kaku4_6wk |
| Sample type |
SRA |
| |
|
| Source name |
6-week-old leaves
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: 6-week-old leaves
genotype: kaku4-2
chip antibody: H3K27me3
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
A native chromatin immunoprecipitation (ChIP) was performed using antibody of histone modification essentially as described with minor modification (Nagaki et al. 2003).
ChIP-seq libraries were constructed by Shanghai Jiayin Biotechnology Co., Ltd and sequenced using the Illumina system Novaseq 6000 with a 150-bp read length.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
All clean ChIP-seq reads were mapped to the Arabidopsis genome TAIR10 using BOWTIE2 software with default parameters. The MACS program was used to shift the reads to identify peaks.
Assembly: Arabidopsis reference genome (TAIR10)
Supplementary files format and content: wig files
|
| |
|
| Submission date |
Dec 15, 2023 |
| Last update date |
Feb 09, 2024 |
| Contact name |
Zhen Su |
| E-mail(s) |
zhensu@cau.edu.cn
|
| Phone |
+86-10-62734385
|
| Organization name |
China Agricultural University
|
| Department |
College of Biological Sciences
|
| Street address |
#2 Yuanmingyuan Xilu
|
| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
| |
|
| Platform ID |
GPL26208 |
| Series (1) |
| GSE250299 |
Transcriptome and epigenome data analysis to reveal the key role of KAKU4 during leaf senescence in Arabidopsis |
|
| Relations |
| BioSample |
SAMN38859573 |
| SRA |
SRX22906800 |
