|
| Status |
Public on Mar 15, 2012 |
| Title |
Rhodopseudomonas palustris cells NF with succinate and yeast extract #2 |
| Sample type |
RNA |
| |
|
| Source name |
Rhodopseudomonas palustris
|
| Organism |
Rhodopseudomonas palustris |
| Characteristics |
strain: CGA010
growth: NF with succinate and yeast extract
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rhodopseudomonas palustris cells were disrupted by bead beating and RNA was purified with the RNeasy Mini kit (Qiagen) including DNase treatment on the column.
|
| Label |
Biotinylated GeneChip reagent
|
| Label protocol |
As recommended by the manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
As recommended by the manufacturer (Affymetrix)
|
| Scan protocol |
As recommended by the manufacturer (Affymetrix)
|
| Description |
A high-density oligonucleotide microarray (Affymetrix GeneChip) was custom designed and manufactured by Affymetrix based on the sequences of ORFs and intergenic regions. cDNA synthesis, fragmentation, labeling, hybridization, and processing of custom designed Rhodopseudomonas palustris GeneChips were carried out as recommended by the manufacturer. Experiments were performed in duplicate with independently prepared samples.
|
| Data processing |
The Affymetrix GeneChip Operating Software Version 1.4 was used for initial data acquisition and processing.
|
| |
|
| Submission date |
Sep 19, 2011 |
| Last update date |
Mar 15, 2012 |
| Contact name |
Yasuhiro Oda |
| E-mail(s) |
yasuhiro@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Department of Microbiology
|
| Lab |
Harwood
|
| Street address |
Box 357242, 1705 N.E. Pacific Street, HSB K-354
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195-7242 |
| Country |
USA |
| |
|
| Platform ID |
GPL3954 |
| Series (1) |
| GSE32292 |
How post-translational modification of nitrogenase is circumvented in Rhodopseudomonas palustris strains that produce hydrogen gas constitutively |
|
