|
| Status |
Public on Dec 31, 2011 |
| Title |
Bovine adrenocortical cells transduced with Ras and p53, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Primary bovine adrenocortical cells
|
| Organism |
Bos taurus |
| Characteristics |
tissue: adrenal cortex
transducer: Ras and p53
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Rneasy kit
|
| Label |
biotin
|
| Label protocol |
One microgram of total RNA was amplified and biotin-labeled using GeneChip Expression 3' Amplification one-Cycle Target Labeling and Control Reagents kit
|
| |
|
| Hybridization protocol |
Following fragmentation, 10ug of labeled cRNA were hybridized for 16 hours at 45C on Affymetrix GeneChip Bovine Genome Array. Arrays were washed and stained with streptavidin-phycoerythrin in a fluidic 450 (Affymetrix).
|
| Scan protocol |
The arrays were read with a confocal laser (GeneChip scanner 3000, Affymetrix).
|
| Description |
Gene expression from cells transduced first by oncogenic Ras followed by p53
|
| Data processing |
The obtained data were normalized with Affymetrix Expression Console software using MAS5 statistical algorithm.Normalized data were compared and filtered using partek Genomic Suite software 6.5.
|
| |
|
| Submission date |
Sep 22, 2011 |
| Last update date |
Dec 31, 2011 |
| Contact name |
Michaël Thomas |
| E-mail(s) |
michael.thomas@cea.fr
|
| Phone |
(33)-438-78-44-64
|
| Fax |
(33)-438-78-50-58
|
| Organization name |
Inserm U1036
|
| Department |
iRTSV/BCI
|
| Street address |
17 rue des martyrs
|
| City |
Grenoble |
| ZIP/Postal code |
38054 |
| Country |
France |
| |
|
| Platform ID |
GPL2112 |
| Series (1) |
| GSE32305 |
Establishing a set of genes differentially expressed in benign versus malignant adrenocortical cells |
|
