|
Status |
Public on Feb 27, 2024 |
Title |
TF ChIP-seq from whole organism (ENCLB529LGA) |
Sample type |
SRA |
|
|
Source name |
Drosophila melanogaster strain Hsf-GFP mixed sex embryo (0-24 hours) insertion targeting D. melanogaster Hsf
|
Organism |
Drosophila melanogaster |
Characteristics |
antibody: Hsf cell_type: whole organism biomaterial_type: whole organisms biosample encode accession: ENCBS682WYO (SAMN40087545) collection date: not applicable geographic location: not applicable encode accession: ENCBS682WYO note: Transgenic D. mel line, stably expressing a C-terminal GFP fused to Hsf Sex: pooled male and female dev stage: embryonic age: 0-24 hour donor_id: ENCDO056OQE lab: Kevin White, UChicago source: Kevin White link: ENCBS682WYO at ENCODE; https://www.encodeproject.org/ENCBS682WYO/
|
Extracted molecule |
genomic DNA |
Extraction protocol |
general protocol: https://www.encodeproject.org/documents/47d3fb40-f2e5-427a-9e29-501dfdaf41fb/@@download/attachment/modENCODE_protocols_Column_SPRI.pdf other: https://www.encodeproject.org/documents/645ec5ec-7287-4b0f-9cbe-d1edd4a09bc1/@@download/attachment/2014-2487.pdf
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
https://www.encodeproject.org/experiments/ENCSR189NRY/ *************** biological replicate number: 3 technical replicate number: 1 description: ChIP-seq on transgenic flies expressing Hsf-eGFP fusion proteins. The IP was performed using an anti-GFP antibody. experiment encode accession: ENCSR189NRY assay title: TF ChIP-seq assembly: dm6, dm3 possible controls: ENCSR768IJB encode release date: 2016-07-06 lab: Kevin White, UChicago library encode accession: ENCLB529LGA fragmentation methods: sonication (Bioruptor generic) size range: 200-800
|
Data processing |
See GSM*_README.txt supplementary file linked below
|
|
|
Submission date |
Feb 24, 2024 |
Last update date |
Feb 27, 2024 |
Contact name |
ENCODE DCC |
E-mail(s) |
encode-help@lists.stanford.edu
|
Organization name |
ENCODE DCC
|
Street address |
300 Pasteur Dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305-5120 |
Country |
USA |
|
|
Platform ID |
GPL13304 |
Series (1) |
GSE256578 |
TF ChIP-seq from whole organism (ENCSR189NRY) |
|
Relations |
BioSample |
SAMN40087545 |
Supplementary file |
Size |
Download |
File type/resource |
GSM8106182_ENCFF067QPD_control_normalized_signal_dm3.bigWig |
15.2 Mb |
(ftp)(http) |
BIGWIG |
GSM8106182_ENCFF162CAZ_read-depth_normalized_signal_dm3.bigWig |
14.5 Mb |
(ftp)(http) |
BIGWIG |
GSM8106182_ENCFF275TGT_signal_of_unique_reads_dm3.bigWig |
14.6 Mb |
(ftp)(http) |
BIGWIG |
GSM8106182_ENCFF282LZL_read-depth_normalized_signal_dm6.bigWig |
16.6 Mb |
(ftp)(http) |
BIGWIG |
GSM8106182_ENCFF308EVZ_signal_of_unique_reads_dm6.bigWig |
16.6 Mb |
(ftp)(http) |
BIGWIG |
GSM8106182_ENCFF498SDV_control_normalized_signal_dm6.bigWig |
16.5 Mb |
(ftp)(http) |
BIGWIG |
GSM8106182_ENCFF549SEJ_peaks_and_background_as_input_for_IDR_dm3.bed.gz |
670.9 Kb |
(ftp)(http) |
BED |
GSM8106182_ENCFF593RDD_peaks_and_background_as_input_for_IDR_dm6.bed.gz |
745.5 Kb |
(ftp)(http) |
BED |
GSM8106182_ENCFF812AVS_peaks_and_background_as_input_for_IDR_dm6.bigBed |
1.2 Mb |
(ftp)(http) |
BIGBED |
GSM8106182_ENCFF917EKN_peaks_and_background_as_input_for_IDR_dm3.bigBed |
1.1 Mb |
(ftp)(http) |
BIGBED |
GSM8106182_ENCLB529LGA_README.txt.gz |
1.9 Kb |
(ftp)(http) |
TXT |
Raw data are available in SRA |