|
Status |
Public on Feb 27, 2024 |
Title |
TF ChIP-seq from whole organism (ENCLB925OFY) |
Sample type |
SRA |
|
|
Source name |
Drosophila melanogaster strain cyc-GFP mixed sex prepupa at white prepupa stage insertion targeting D. melanogaster cyc
|
Organism |
Drosophila melanogaster |
Characteristics |
antibody: cyc cell_type: whole organism biomaterial_type: whole organisms biosample encode accession: ENCBS859CWX (SAMN40087943) collection date: not applicable geographic location: not applicable encode accession: ENCBS859CWX note: Transgenic D. mel line, stably expressing a C-terminal GFP fused to cyc Sex: pooled male and female dev stage: prepupa age: unknown donor_id: ENCDO848KXI lab: Kevin White, UChicago source: Kevin White fly synchronization stage: white prepupa link: ENCBS859CWX at ENCODE; https://www.encodeproject.org/ENCBS859CWX/
|
Extracted molecule |
genomic DNA |
Extraction protocol |
general protocol: https://www.encodeproject.org/documents/47d3fb40-f2e5-427a-9e29-501dfdaf41fb/@@download/attachment/modENCODE_protocols_Column_SPRI.pdf other: https://www.encodeproject.org/documents/68208eeb-8445-47e6-8d88-7dbe3913bf2f/@@download/attachment/2014-2456.pdf
|
|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
https://www.encodeproject.org/experiments/ENCSR007HUT/ *************** biological replicate number: 2 technical replicate number: 1 description: ChIP-seq on transgenic flies expressing cyc-eGFP fusion proteins. The IP was performed using an anti-GFP antibody. experiment encode accession: ENCSR007HUT assay title: TF ChIP-seq assembly: dm6, dm3 possible controls: ENCSR807ABQ encode release date: 2016-07-06 lab: Kevin White, UChicago library encode accession: ENCLB925OFY fragmentation methods: sonication (Bioruptor generic) size range: 200-800
|
Data processing |
See GSM*_README.txt supplementary file linked below
|
|
|
Submission date |
Feb 24, 2024 |
Last update date |
Feb 27, 2024 |
Contact name |
ENCODE DCC |
E-mail(s) |
encode-help@lists.stanford.edu
|
Organization name |
ENCODE DCC
|
Street address |
300 Pasteur Dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305-5120 |
Country |
USA |
|
|
Platform ID |
GPL13304 |
Series (1) |
GSE256592 |
TF ChIP-seq from whole organism (ENCSR007HUT) |
|
Relations |
BioSample |
SAMN40087943 |
Supplementary file |
Size |
Download |
File type/resource |
GSM8106207_ENCFF149UIM_control_normalized_signal_dm6.bigWig |
16.4 Mb |
(ftp)(http) |
BIGWIG |
GSM8106207_ENCFF227PTC_read-depth_normalized_signal_dm6.bigWig |
16.3 Mb |
(ftp)(http) |
BIGWIG |
GSM8106207_ENCFF299QWE_peaks_and_background_as_input_for_IDR_dm3.bigBed |
1.2 Mb |
(ftp)(http) |
BIGBED |
GSM8106207_ENCFF301RWV_peaks_and_background_as_input_for_IDR_dm3.bed.gz |
730.9 Kb |
(ftp)(http) |
BED |
GSM8106207_ENCFF406KCK_signal_of_unique_reads_dm3.bigWig |
14.3 Mb |
(ftp)(http) |
BIGWIG |
GSM8106207_ENCFF515OLF_control_normalized_signal_dm3.bigWig |
15.0 Mb |
(ftp)(http) |
BIGWIG |
GSM8106207_ENCFF520ETR_signal_of_unique_reads_dm6.bigWig |
16.3 Mb |
(ftp)(http) |
BIGWIG |
GSM8106207_ENCFF593QCA_peaks_and_background_as_input_for_IDR_dm6.bigBed |
1.2 Mb |
(ftp)(http) |
BIGBED |
GSM8106207_ENCFF678YPX_peaks_and_background_as_input_for_IDR_dm6.bed.gz |
729.7 Kb |
(ftp)(http) |
BED |
GSM8106207_ENCFF906TXF_read-depth_normalized_signal_dm3.bigWig |
14.3 Mb |
(ftp)(http) |
BIGWIG |
GSM8106207_ENCLB925OFY_README.txt.gz |
1.9 Kb |
(ftp)(http) |
TXT |
Raw data are available in SRA |