|
Status |
Public on Feb 27, 2024 |
Title |
TF ChIP-seq from whole organism (ENCLB807FCF) |
Sample type |
SRA |
|
|
Source name |
Caenorhabditis elegans strain RW12226 (st12226[pbrm-1::TY1::EGFP::3xFLAG] I) young adult (64.5 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting C. elegans pbrm-1
|
Organism |
Caenorhabditis elegans |
Characteristics |
antibody: pbrm-1 cell_type: whole organism biomaterial_type: whole organisms biosample encode accession: ENCBS597RGR (SAMN40085298) collection date: not applicable geographic location: not applicable encode accession: ENCBS597RGR Sex: not collected dev stage: young adult age: 64.5 hour donor_id: ENCDO223KDW lab: Valerie Reinke, Yale source: Valerie Reinke worm synchronization stage: L1 larva starved after bleaching link: ENCBS597RGR at ENCODE; https://www.encodeproject.org/ENCBS597RGR/
|
Extracted molecule |
genomic DNA |
Extraction protocol |
general protocol: https://www.encodeproject.org/documents/d89f19f3-31f8-4079-9635-5d3dd799471e/@@download/attachment/Reinke_Lab_ModENCODE_ChIP_protocol_%28revised%205-24-13%29_4_mg.pdf
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|
|
Library strategy |
ChIP-Seq |
Library source |
genomic |
Library selection |
ChIP |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
https://www.encodeproject.org/experiments/ENCSR111IAZ/ *************** biological replicate number: 1 technical replicate number: 1 description: ChIP-seq on transgenic worms expressing pbrm-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody. experiment encode accession: ENCSR111IAZ assay title: TF ChIP-seq assembly: ce11 possible controls: ENCSR868TYN encode release date: 2022-11-01 lab: Valerie Reinke, Yale library encode accession: ENCLB807FCF fragmentation methods: sonication (Bioruptor generic) size range: 200-800
|
Data processing |
See GSM*_README.txt supplementary file linked below
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|
|
Submission date |
Feb 24, 2024 |
Last update date |
Feb 27, 2024 |
Contact name |
ENCODE DCC |
E-mail(s) |
encode-help@lists.stanford.edu
|
Organization name |
ENCODE DCC
|
Street address |
300 Pasteur Dr
|
City |
Stanford |
State/province |
CA |
ZIP/Postal code |
94305-5120 |
Country |
USA |
|
|
Platform ID |
GPL26672 |
Series (1) |
GSE256615 |
TF ChIP-seq from whole organism (ENCSR111IAZ) |
|
Relations |
BioSample |
SAMN40085298 |