|
| Status |
Public on Jul 26, 2024 |
| Title |
E25 Pantherophis guttatus dorsal skin single cells |
| Sample type |
SRA |
| |
|
| Source name |
Embryonic dorsal skin
|
| Organism |
Pantherophis guttatus |
| Characteristics |
tissue: Embryonic dorsal skin
cell type: Skin-resident cells
genotype: Wild Type +/+
treatment: None
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Dorsal skin was extracted from one Pantherophis guttatus embryo at 25 days post-ovipositioning (E25). The dorsal skin was then processed according to the protocol detailed in “Dissociation of chick embryonic tissue for FACS and preparation of isolated cells for genome-wide downstream assays” by Williams et al., 2021. The resulting single cell suspension was frozen at -80°C and sent for library preparation followed by sequencing.
10X Genomics Single Cell 3' v3 performed by Single Cell Discoveries, subsequent sequencing was performed by Macrogen.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
10X Genomics Single Cell 3' v3 library
|
| Data processing |
The demultiplexing, barcoded processing, gene counting and aggregation were made using Cell Ranger software v7.0.0
Assembly: UNIGE_PanGut_3.0
Supplementary files format and content: h5ad file with count matrices, scores and clustering
|
| |
|
| Submission date |
Mar 21, 2024 |
| Last update date |
Jul 26, 2024 |
| Contact name |
Athanasia Tzika |
| Organization name |
University of Geneva
|
| Street address |
Athanasia Tzika, LANE - Sciences III, 30 quai Ernest Ansermet, 1211 Genève 4, Switzerland
|
| City |
Geneva |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL34323 |
| Series (1) |
| GSE262160 |
PMEL is involved in snake color pattern transition from blotches to stripes [scRNA-seq] |
|
| Relations |
| BioSample |
SAMN40568857 |
| SRA |
SRX24016433 |
