|
| Status |
Public on May 13, 2024 |
| Title |
Day 2-VC-M2 |
| Sample type |
SRA |
| |
|
| Source name |
Whole
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: Whole
genotype: WT
treatment: Inhalation, Filtered air
|
| Treatment protocol |
The flies of both sexes were exposed to CMIT/MIT aerosol for 1 hour at 330 mg/m3.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent (Invitrogen). 500 ng of total RNA was used for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using LEXOGEN Quant-Seq Library Prep Kit (Cat#015) standard protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Data processing |
Illumina Casava1.8 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and filtered for low-complexity or low-quality sequence(using fastp), then mapped to dm6 (NCBI) whole genome using STAR.
Read count extraction using HTSeq-count.
Assembly: dm6
Supplementary files format and content: tab-delimited text files include read count values for each Sample
|
| |
|
| Submission date |
Apr 09, 2024 |
| Last update date |
May 13, 2024 |
| Contact name |
Yoon Cho |
| E-mail(s) |
yoon.cho@kitox.re.kr
|
| Organization name |
Korea Institute of Toxiology
|
| Street address |
30, Baekhak 1-gil
|
| City |
Jeongeup |
| State/province |
Jeollabuk-do |
| ZIP/Postal code |
56212 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL22106 |
| Series (1) |
| GSE263572 |
Inhalation exposure to CMIT/MIT in Drosophila melanogaster |
|
| Relations |
| BioSample |
SAMN40901925 |
| SRA |
SRX24200669 |
