|
| Status |
Public on Apr 12, 2024 |
| Title |
Cytotrophoblast mock infected, biol rep 1 |
| Sample type |
SRA |
| |
|
| Source name |
Trophoblast cells
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Trophoblast cells
cell line: Trophoblast stem cell (clone 27)
cell type: Cytotrophoblast
treatment: Trophoblast stem cell media
|
| Growth protocol |
Trophoblast stem cells were seeded at a ratio of 1.5 x 10^5 in 6-well plates and induced to form syncytiotrophoblast cells using a differentiation medium. After 5 days of differentiation, cells were infected or mock-infected with T. gondii RH: YFP (5 parasites/cell). For comparison, cytotrophoblast cells were seeded in a 6-well plate and infected or mock-infected with T. gondii at the same MOI used for syncytiotrophoblast cells.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Qiagen Rneasy
RNA libraries for RNA-seq were prepared using NEBNext Ultra II Directional RNA Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Description |
RS_01_S1
|
| Data processing |
CLC Genomics Workbench v 23.0
Assembly: Read libraries were mapped to the human (hg38) transcriptomes using CLC Genomics Workbench.
Supplementary files format and content: tab-delimited text files include raw counts for each sample
|
| |
|
| Submission date |
Apr 09, 2024 |
| Last update date |
Apr 19, 2024 |
| Contact name |
Rafaela Jose da Silva |
| E-mail(s) |
rjs224@pitt.edu
|
| Phone |
(412) 214-3649
|
| Organization name |
University of Pittburgh
|
| Department |
Biological Sciences
|
| Street address |
4249 Fifth Avenue
|
| City |
Pittsburgh |
| State/province |
Pennsylvania |
| ZIP/Postal code |
15260 |
| Country |
USA |
| |
|
| Platform ID |
GPL30173 |
| Series (1) |
| GSE263615 |
Transcriptome analysis of cytotrophoblasts and syncytiotrophoblasts derived from trophoblast stem cells, mock-infected or infected with Toxoplasma gondii |
|
| Relations |
| BioSample |
SAMN40906535 |
| SRA |
SRX24203185 |
