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| Status |
Public on Apr 12, 2024 |
| Title |
Transcriptome analysis of cytotrophoblasts and syncytiotrophoblasts derived from trophoblast stem cells, mock-infected or infected with Toxoplasma gondii |
| Organisms |
Toxoplasma gondii; Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We tested the relevance of an established stem-cell-derived model of trophoblast development to Toxoplasma gondii infection. Human trophoblast stem cells were cultured under conditions suitable for cytotrophoblast cells or for the differentiation into syncytiotrophoblasts, and subsequently infected with T. gondii. RNA-seq data from both mock-treated and infected cells revealed differences between cell types and their respective responses to T. gondii infection.
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| Overall design |
Human trophoblast stem cells were cultured as cytotrophoblast cells and also differentiated into syncytiotrophoblast using a differentiation medium. Cells were mock-infected or infected with T. gondii (RH-YFP strain) with a multiplicity of infection (MOI) of 5. RNA-seq was performed in order to evaluate the differences in transcriptome between both cells and the changes after infection. The experiment was designed with 3 biological replicates for each condition.
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| Web link |
https://pubmed.ncbi.nlm.nih.gov/38771057/
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| Citation(s) |
38771057 |
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| Submission date |
Apr 09, 2024 |
| Last update date |
Aug 21, 2024 |
| Contact name |
Rafaela Jose da Silva |
| E-mail(s) |
rjs224@pitt.edu
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| Phone |
(412) 214-3649
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| Organization name |
University of Pittburgh
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| Department |
Biological Sciences
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| Street address |
4249 Fifth Avenue
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| City |
Pittsburgh |
| State/province |
Pennsylvania |
| ZIP/Postal code |
15260 |
| Country |
USA |
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| Platforms (2) |
| GPL30173 |
NextSeq 2000 (Homo sapiens) |
| GPL34372 |
NextSeq 2000 (Homo sapiens; Toxoplasma gondii) |
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| Samples (12)
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| GSM8195458 |
Cytotrophoblast mock infected, biol rep 1 |
| GSM8195459 |
Cytotrophoblast mock infected, biol rep 2 |
| GSM8195460 |
Cytotrophoblast mock infected, biol rep 3 |
| GSM8195461 |
Cytotrophoblast infected, biol rep 1 |
| GSM8195462 |
Cytotrophoblast infected, biol rep 2 |
| GSM8195463 |
Cytotrophoblast infected, biol rep 3 |
| GSM8195464 |
Syncytiotrophoblast mock infected, biol rep 1 |
| GSM8195465 |
Syncytiotrophoblast mock infected, biol rep 2 |
| GSM8195466 |
Syncytiotrophoblast mock infected, biol rep 3 |
| GSM8195467 |
Syncytiotrophoblast infected, biol rep 1 |
| GSM8195468 |
Syncytiotrophoblast infected, biol rep 2 |
| GSM8195469 |
Syncytiotrophoblast infected, biol rep 3 |
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| Relations |
| BioProject |
PRJNA1098408 |
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