|
| Status |
Public on Apr 29, 2024 |
| Title |
Amniotic fluid cells from Homo sapiens Case No. 4 |
| Sample type |
genomic |
| |
|
| Source name |
amniotic fluid fetal cells
|
| Organism |
Homo sapiens |
| Characteristics |
tag: cell type: amniotic fluid fetal cells diagnosis: microdeletion of 16p11.2
|
| Treatment protocol |
not applicable
|
| Growth protocol |
not applicable
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA of amniotic fluid cells or peripheral blood sampling of the parents was extracted with using the QIAamp DNA Blood Mini Kit (Qiagen, Inc., Hilden, Germany) according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
Labelling and hybridizations were performed according to the manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
DNA was restriction digested, PCR amplified, fragmented, labeled and hybridized to each array according to the manufacturer's instructions.
|
| Scan protocol |
The arrays were then washed using Affymetrix Fluidics Stations, and scanned using the GeneChip System 3000Dx v.2.
|
| Description |
Amniotic fluid cells from Homo sapiens
Increased nuchal translucency (9.9 mm)
|
| Data processing |
ChAS console from Affymetrix was used to assess genome wide copy number frequencies. Log2 Ratio copy number values with associated chromosome positions are available on the series record.
Per marker Log2 Ratio of normalized intensity with respect to a reference, with further correction for sample specific variation.
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| |
|
| Submission date |
Apr 25, 2024 |
| Last update date |
Apr 29, 2024 |
| Contact name |
Hongguo Zhang |
| E-mail(s) |
zhanghguo@jlu.edu.cn
|
| Organization name |
First Hospital, Jilin University, Changchun, China
|
| Department |
Center for Reproductive Medicine and Center for Prenatal Diagnosis
|
| Street address |
1 Xinmin Street, Changchun, Jilin 130021, P.R. China
|
| City |
Changchun |
| State/province |
Jilin |
| ZIP/Postal code |
130021 |
| Country |
China |
| |
|
| Platform ID |
GPL18637 |
| Series (1) |
| GSE265911 |
Prenatal phenotypes and pregnancy outcomes of fetuses with 16p11.2 microdeletion/microduplication |
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