|
| Status |
Public on Jul 25, 2024 |
| Title |
sciatic nerve, BM1 (circRNA) |
| Sample type |
SRA |
| |
|
| Source name |
Rattus sciatic nerve
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Rattus sciatic nerve
condition: rat sciatic nerve deficit injury model
treatment: Bone marrow-derived mesenchymal stem cell transplantation
|
| Treatment protocol |
In BMSCs and ADSCs therapy groups, a pre-digested cell suspension of 2 × 106/mL of P3-generation BMSCs and ADSCs was injected into the entire 10-mm ANA segment using an insulin syringe.
|
| Growth protocol |
Sprague-Dawley rats was fed in an environment with a 12:12h light/dark cycle at controlled temperature and humidity, with free access to food and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted and purified from each sample in each group using Trizol, and agarose gel electrophoresis was utilized to analyze the integrity of RNA and the presence of DNA contamination.
RNA was isolated using TRIzol reagent, and RNA integrity was evaluated using the Agilent 2100 Bioanalyze.
RNA was isolated using TRIzol reagent and NEBNext® Ultra™ RNA Library Prep Kit for Illumina® was used for the construction of sequencing libraries.
RNA-seq( long noncoding RNAs (lncRNAs), microRNAs (miRNAs), and circular RNAs (circRNAs ))
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Agilent 2100 Bioanalyze.
Trimmed sequence reads were mapped to mapped to reference Genome
Novel Transcript prediction and Structure analysis
Differential expression analysis and functional analysis.
Assembly: rn7
Supplementary files format and content: genes.readcount.txt
|
| |
|
| Submission date |
May 10, 2024 |
| Last update date |
Jul 25, 2024 |
| Contact name |
Xiangling Li |
| E-mail(s) |
lixiangling5910@126.com
|
| Phone |
13508285725
|
| Organization name |
Chinese PLA General Hospital
|
| Street address |
Fucheng Road
|
| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100853 |
| Country |
China |
| |
|
| Platform ID |
GPL25947 |
| Series (1) |
| GSE266596 |
Crosstalk between neural tissue-specific extracellular matrix and mesenchymal stem cells and their promotion of peripheral nerve regeneration in vivo |
|
| Relations |
| BioSample |
SAMN41329404 |
| SRA |
SRX24522161 |
