|
| Status |
Public on May 22, 2024 |
| Title |
siNFAT5 under High Na condition |
| Sample type |
RNA |
| |
|
| Source name |
HeLa cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: treatment: siNFAT5
|
| Treatment protocol |
HeLa cells were transfected with siControl, siNFAT5 and siHES1 using lipofectamine RNAiMax.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using Isogen (Wako, Cat#319-90211).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared from total RNA using GeneChip WT PLUS Reagent Kit (Affymetrix) following the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
single stranded cDNA was hybridized for 16 hr at 45˚C on Clariom S Human Array Thermo Fisher Scientific).
|
| Scan protocol |
Arrays were washed and stained in the GeneChip Fluidics Station 450 (Affymetrix) and were scanned using GeneChip Scanner 3000 7G.
|
| Description |
RMA expression value derived from Affymetrix Expression Console Software.
|
| Data processing |
Gene expression data were analyzed with Affymetrix Expression Console Software 1.4.1 offered SST-RMA using Affymetrix default analysis settings.
|
| |
|
| Submission date |
May 16, 2024 |
| Last update date |
May 22, 2024 |
| Contact name |
Hiroki Ryuno |
| Organization name |
The University of Tokyo
|
| Department |
Graduate School of Pharmaceutical Sciences
|
| Lab |
Laboratory of Cell Signaling
|
| Street address |
7-3-1
|
| City |
Hongo, Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE267676 |
Gene expression data from HeLa cells under high salt condition |
|
