|
| Status |
Public on May 25, 2024 |
| Title |
Hg_4-cell_rep5 |
| Sample type |
SRA |
| |
|
| Source name |
whole organism
|
| Organism |
Heterodera glycines |
| Characteristics |
tissue: whole organism
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested by treating eggs with lysis buffer. Details can refer to smart-seq2 peotocols.
RNA libraries for RNA-seq were prepared using Smart-seq2 protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
sequence read was trimmed for adaptor sequence/low-quality using fastp (v0.20.1) software
read count extraction and normalization were performed using RSEM29 (v1.2.28)
Assembly: The genome used in this study was sequenced and assembled in our own laboratory and has not yet been published.
Supplementary files format and content: tab-delimited text file includes raw counts for each Sample
|
| |
|
| Submission date |
May 21, 2024 |
| Last update date |
May 25, 2024 |
| Contact name |
Dadong Dai |
| E-mail(s) |
daidadong@mail.hzau.edu.cn
|
| Organization name |
Huazhong Agricultural University
|
| Department |
Department of Life science and technology
|
| Lab |
BMB Lab
|
| Street address |
1st street Shizishan
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL34499 |
| Series (1) |
| GSE268038 |
Plant parasitic nematodes single embryo RNA sequencing |
|
| Relations |
| BioSample |
SAMN36271153 |
| SRA |
SRX20871803 |
