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Series GSE268038 Query DataSets for GSE268038
Status Public on May 25, 2024
Title Plant parasitic nematodes single embryo RNA sequencing
Organisms Meloidogyne incognita; Heterodera glycines; Ditylenchus destructor
Experiment type Expression profiling by high throughput sequencing
Summary In order to study the similarities and differences in embryonic development between plant-parasitic nematodes and free-living nematodes, we performed RNA-seq on embryos of three plant-parasitic nematodes at a total of 11 stages from the single-cell stage to the J1 stage
 
Overall design We recorded the embryonic development process of Meloidogyne incognita, Heterodera glycines, and Ditylenchus destructor species, and constructed transcriptome datasets of single embryos of these three species utilized low-input smart-seq2 RNA-seq technology. The datasets encompassed 11 complete embryonic development stages, which are Zygote, 2-cell, 4-cell, 8-cell, 24-44 cell, 64-78 cell, Comma, 1.5-fold, 2-fold, Moving, L1, which each stage generated four to five replicates. a total of 162 high-resolution transcriptome libraries were obtained.
 
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BioProject PRJNA990458
Submission date May 21, 2024
Last update date May 25, 2024
Contact name Dadong Dai
E-mail(s) daidadong@mail.hzau.edu.cn
Organization name Huazhong Agricultural University
Department Department of Life science and technology
Lab BMB Lab
Street address 1st street Shizishan
City Wuhan
State/province Hubei
ZIP/Postal code 430070
Country China
 
Platforms (3)
GPL34498 Illumina NovaSeq 6000 (Ditylenchus destructor)
GPL34499 Illumina NovaSeq 6000 (Heterodera glycines)
GPL34500 Illumina NovaSeq 6000 (Meloidogyne incognita)
Samples (162)
GSM8284522 Dd_Zygote_rep1
GSM8284523 Dd_Zygote_rep2
GSM8284524 Dd_Zygote_rep3

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE268038_Dd_raw_counts.txt.gz 1.1 Mb (ftp)(http) TXT
GSE268038_Hg_raw_counts.txt.gz 1.2 Mb (ftp)(http) TXT
GSE268038_Mi_raw_counts.txt.gz 2.4 Mb (ftp)(http) TXT
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