|
| Status |
Public on May 03, 2012 |
| Title |
set3_Col_30min_flg |
| Sample type |
RNA |
| |
|
| Source name |
Arabidopsis_Col_flg22_30min
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col
genotype: wildtype
treatment: flg22
time: 30min
|
| Treatment protocol |
Arabidopsis seedlings were treated with 1uM flg22 or water
|
| Growth protocol |
Ten-day-old Arabidopsis seedlings were grown in MS medium under a 12h dark/12h light regime at 22°C and transferred to multi-well plates containing fresh liquid MS media 48 h before flg22 (1uM) or mock (water) treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from leaves was isolated using the Trizol Reagent method according to the manufacturer’s recommendations (Invitrogen)
|
| Label |
Alexa555
|
| Label protocol |
Labeling was performed as described in Sato et al., The Plant Journal (2007) 49: 565-577
|
| |
|
| Hybridization protocol |
Hybridization was performed as described in Sato et al., The Plant Journal (2007) 49: 565-577
|
| Scan protocol |
Scanning was performed as described in Sato et al., The Plant Journal (2007) 49: 565-577
|
| Description |
All spots on the microrray include DNA fragments which binds to Cy5 labeled oligo DNA so that spots with bad quality can be identified and removed from further analysis.
|
| Data processing |
The scanned data were subjected to quantile normalization as described in Sato et al., The Plant Journal (2007) 49: 565-577
|
| |
|
| Submission date |
Nov 12, 2011 |
| Last update date |
May 03, 2012 |
| Contact name |
Kenichi Tsuda |
| E-mail(s) |
tsuda@mail.hzau.edu.cn, tsuda@mpipz.mpg.de
|
| Organization name |
Huazhong Agricultural University
|
| Lab |
Kenichi Tsuda
|
| Street address |
No.1 Shizishan Road
|
| City |
Wuhan |
| ZIP/Postal code |
430070 |
| Country |
China |
| |
|
| Platform ID |
GPL3638 |
| Series (1) |
| GSE33648 |
Expression analysis of Arabidopsis mpk11 mutant treated with flg22 |
|
