|
| Status |
Public on Dec 22, 2011 |
| Title |
Whisker pad mutant biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
E12.5 Whisker pad Trps1 mutant
|
| Organism |
Mus musculus |
| Characteristics |
tissue: whole whisker pad
genotype: homozygous Trps1 mutant
age: E12.5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole whisker pads were dissected from E12.5 embryos.
Total RNA was isolated using the RNeasy® Mini Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
15 ug of cRNA were hybridized to Affymetrix GeneChip® MOE430A microarrays.
|
| Scan protocol |
Affymetrix protocol
|
| Description |
Gene expression data during vibrissae placode formation
|
| Data processing |
The data output was analyzed using GeneSpring GX 10.0 commercial software (Agilent Technologies Inc., Palo Alto, CA, USA). p-values were calculated using an unpaired t-test. Expression values with a p-value ≤0.05 and a fold difference of ≥1.5-fold relative to wild-type baseline expression levels were considered significant.
Affymetrix Gene Expression, MOE 430A chips Filter by expression Raw data Filter by percentile Upper cutoff = 100.0 Lower cutoff = 20.0 At least 1 out of 6 samples have values within range T-test unpaired p-value computation: Asymptotic Multiple testing correction: No correction p-value cutoff = 0.05 Fold change cutoff = 1.5 Clustering: Hierarchical Interpretation: A. All Samples B. Genotype Cluster on: Entities and Conditions
|
| |
|
| Submission date |
Nov 17, 2011 |
| Last update date |
Dec 22, 2011 |
| Contact name |
Katherine Fantauzzo |
| E-mail(s) |
kaf2103@columbia.edu
|
| Organization name |
Columbia University
|
| Street address |
1150 St. Nicholas Avenue Room 307
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL339 |
| Series (1) |
| GSE33766 |
Transcriptional targets of Trps1 in vibrissae |
|
