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Sample GSM835602 Query DataSets for GSM835602
Status Public on Jul 24, 2012
Title HF_1h_ctrl, biological rep1
Sample type RNA
 
Source name Pair-fed carbohydrate control liver
Organism Rattus norvegicus
Characteristics tissue: liver
hepatectomy: no
animal id: H-239
diet: High fat
timepoint: 0 (control)
array scan date: 2010-01-28
outlier: no
Treatment protocol Liver tissue was freeze-clamped in liquid nitrogen at the time of partial hepatectomy (PHx) and 1 h, 6 h, 12 h, and 24 h after PHx. Tissue was then stored at -80 degrees C.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was purified using RNeasy mini kit (Qiagen) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100
Label Biotin
Label protocol Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2 ug total RNA.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr on Rat Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol Chips were scanned using Affymetrix GeneChip Scanner 3000.
Description Gene expression in pair-fed carbohydrate control liver
Data processing Raw gene expression data was normalized using RMA in Affymetrix Expression Console.
 
Submission date Nov 17, 2011
Last update date Jul 25, 2012
Contact name Egle Juskeviciute
Organization name Thomas Jefferson University
Department Pathology
Street address 1020 Locust st. JAH 527
City Philadelphia
State/province PA
ZIP/Postal code 19107
Country USA
 
Platform ID GPL6247
Series (1)
GSE33785 Gene expression in the chronic ethanol-treated rat liver during liver regeneration

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity (outliers present, no batch correction)

Data table
ID_REF VALUE
10700001 11.3836
10700002 6.11023
10700003 9.76643
10700004 3.14361
10700005 8.95146
10700006 2.38908
10700007 2.47749
10700008 2.00044
10700009 8.07686
10700010 2.6455
10700011 3.74591
10700012 3.15952
10700013 10.4689
10700014 9.7175
10700015 8.24203
10700016 2.37129
10700017 5.20932
10700018 2.33019
10700019 2.41566
10700020 11.5454

Total number of rows: 29214

Table truncated, full table size 481 Kbytes.




Supplementary file Size Download File type/resource
GSM835602.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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