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Sample GSM835625 Query DataSets for GSM835625
Status Public on Jul 24, 2012
Title HF_24h_phx, biological rep4
Sample type RNA
 
Source name Pair-fed carbohydrate control liver 24h after PHx
Organism Rattus norvegicus
Characteristics tissue: liver
hepatectomy: yes
animal id: 3H-29
diet: High fat
timepoint: 24 hours
array scan date: 2009-12-01
outlier: no
Treatment protocol Liver tissue was freeze-clamped in liquid nitrogen at the time of partial hepatectomy (PHx) and 1 h, 6 h, 12 h, and 24 h after PHx. Tissue was then stored at -80 degrees C.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was purified using RNeasy mini kit (Qiagen) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100
Label Biotin
Label protocol Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2 ug total RNA.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr on Rat Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol Chips were scanned using Affymetrix GeneChip Scanner 3000.
Description Gene expression in pair-fed carbohydrate control liver 24h after PHx
Data processing Raw gene expression data was normalized using RMA in Affymetrix Expression Console.
 
Submission date Nov 17, 2011
Last update date Jul 25, 2012
Contact name Egle Juskeviciute
Organization name Thomas Jefferson University
Department Pathology
Street address 1020 Locust st. JAH 527
City Philadelphia
State/province PA
ZIP/Postal code 19107
Country USA
 
Platform ID GPL6247
Series (1)
GSE33785 Gene expression in the chronic ethanol-treated rat liver during liver regeneration

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity (outliers present, no batch correction)

Data table
ID_REF VALUE
10700001 10.9905
10700002 4.82016
10700003 9.28529
10700004 3.03862
10700005 8.54506
10700006 2.24704
10700007 2.31974
10700008 2.17835
10700009 6.20452
10700010 2.4692
10700011 3.19951
10700012 2.89657
10700013 10.2436
10700014 9.20081
10700015 6.52693
10700016 2.30619
10700017 4.1547
10700018 2.19808
10700019 2.29923
10700020 10.2341

Total number of rows: 29214

Table truncated, full table size 481 Kbytes.




Supplementary file Size Download File type/resource
GSM835625.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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