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Sample GSM835630 Query DataSets for GSM835630
Status Public on Jul 24, 2012
Title CHO_1h_ctrl, biological rep3
Sample type RNA
 
Source name Pair-fed high fat control liver
Organism Rattus norvegicus
Characteristics tissue: liver
hepatectomy: no
animal id: H-252
diet: Carbohydrate
timepoint: 0 (control)
array scan date: 2010-02-05
outlier: no
Treatment protocol Liver tissue was freeze-clamped in liquid nitrogen at the time of partial hepatectomy (PHx) and 1 h, 6 h, 12 h, and 24 h after PHx. Tissue was then stored at -80 degrees C.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was purified using RNeasy mini kit (Qiagen) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100
Label Biotin
Label protocol Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2 ug total RNA.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr on Rat Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol Chips were scanned using Affymetrix GeneChip Scanner 3000.
Description Gene expression in pair-fed high fat control liver
Data processing Raw gene expression data was normalized using RMA in Affymetrix Expression Console.
 
Submission date Nov 17, 2011
Last update date Jul 25, 2012
Contact name Egle Juskeviciute
Organization name Thomas Jefferson University
Department Pathology
Street address 1020 Locust st. JAH 527
City Philadelphia
State/province PA
ZIP/Postal code 19107
Country USA
 
Platform ID GPL6247
Series (1)
GSE33785 Gene expression in the chronic ethanol-treated rat liver during liver regeneration

Data table header descriptions
ID_REF
VALUE RMA normalized signal intensity (outliers present, no batch correction)

Data table
ID_REF VALUE
10700001 11.4734
10700002 6.16462
10700003 9.88868
10700004 3.04676
10700005 9.22675
10700006 2.4213
10700007 2.56492
10700008 2.45471
10700009 8.45964
10700010 2.69263
10700011 3.7334
10700012 3.25898
10700013 10.6984
10700014 9.8961
10700015 8.60255
10700016 2.41286
10700017 5.35189
10700018 2.49682
10700019 2.48344
10700020 11.5478

Total number of rows: 29214

Table truncated, full table size 481 Kbytes.




Supplementary file Size Download File type/resource
GSM835630.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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