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Status |
Public on Jul 24, 2012 |
Title |
CHO_6h_phx, biological rep4 |
Sample type |
RNA |
|
|
Source name |
Pair-fed high fat control liver 6h after PHx
|
Organism |
Rattus norvegicus |
Characteristics |
tissue: liver hepatectomy: yes animal id: H-285 diet: Carbohydrate timepoint: 6 hours array scan date: 2008-11-12 outlier: no
|
Treatment protocol |
Liver tissue was freeze-clamped in liquid nitrogen at the time of partial hepatectomy (PHx) and 1 h, 6 h, 12 h, and 24 h after PHx. Tissue was then stored at -80 degrees C.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was purified using RNeasy mini kit (Qiagen) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2 ug total RNA.
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|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr on Rat Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
Chips were scanned using Affymetrix GeneChip Scanner 3000.
|
Description |
Gene expression in pair-fed high fat control liver 6h after PHx
|
Data processing |
Raw gene expression data was normalized using RMA in Affymetrix Expression Console.
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|
|
Submission date |
Nov 17, 2011 |
Last update date |
Jul 25, 2012 |
Contact name |
Egle Juskeviciute |
Organization name |
Thomas Jefferson University
|
Department |
Pathology
|
Street address |
1020 Locust st. JAH 527
|
City |
Philadelphia |
State/province |
PA |
ZIP/Postal code |
19107 |
Country |
USA |
|
|
Platform ID |
GPL6247 |
Series (1) |
GSE33785 |
Gene expression in the chronic ethanol-treated rat liver during liver regeneration |
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